Is hypochlorous acid (HOCI) involved in age-related nuclear cataract?

Linda J. Hazell, Hongjie Fu, Roger T. Dean, Roland Stocker, Roger J.W. Truscott

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Oxidative damage to lens proteins is associated with human age-related nuclear cataract and H 2O 2 has been implicated. As hypochlorous acid (HOCI) can be formed from chloride ions and H 2O 2 and many of the protein modifications observed in cataract are also noted following exposure of proteins to HOCI, age-related nuclear cataract lenses were examined for evidence of HOCI-mediated alterations. Method: Three techniques were employed using human lens samples: 1. staining with a HOP antibody that recognises HOCI-treated proteins, 2. myeloperoxidase assays and 3. measurement of chlorotyrosine in acid digests of the crystallins. Results: HOP staining was inconclusive, although cataract lenses appeared to stain more intensely than normals. No myeloperoxidase activity could be detected and neither mono-nor di-chlorotyrosine could be found in human lens proteins. Conclusions: On the basis of this study, no evidence was obtained to support a role for HOCl in age-related nuclear cataract.
Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalClinical and Experimental Optometry
Volume85
Issue number2
DOIs
Publication statusPublished - Mar 2002

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