Ligula intestinalisis a species with a complex biology. Several strains, distinct in the hostspecificity and biogeography, are supposed to exist; however, their morphological or molecular identification remains unresolved. We describe the isolation of 16 polymorphicmicro satellite markers. The number of alleles ranged from four to 14 in a population of 21L. intestinalis individuals. Loci showed amplification success across samples of world wide distribution. A cross-amplification of 14 markers in Digramma interrupta(Cestoda: Pseudophyllidea)was observed.