Natural polymers play a very important role in various pharmaceutical formulations. They are effortlessly accessible, low cost, biodegradable, non-poisonous, non-irritating and may be considered as pharmaceutical excipients. Nowadays investigation is ongoing for finding natural gums and mucilage for diversity of reasons including use in pharmaceutical formulations. The present study is focused on the isolation of mucilage from Tinospora cordifolia and characterization for use as pharmaceutical binder and release modifier. The tablets were prepared by wet granulation process using four different compositions of mucilage (1%, 2.5%, 5%, 10% w/w) alone and in combination with potato starch (10% w/w). The tablets were characterized for pre and post pressure parameters, like, angle of repose, tapped density, bulk density, Carr’s index, Hausner’s ratio, friability and hardness, variation of weight, disintegration time, content uniformity and studies of in vitro drug release. It was found that release of drug from the tablet decreased (3 and 5 h) with increase in concentration of mucilage (5 and 10% w/w) and found sustained release of drug from the tablet for more than 6 hours at higher concentration of mucilage combined with starch (10% w/w). The result shows the potentials of Tinospora cardifolia mucilage for use as binder for the preparation of uncoated tablet and for sustained release drug formulation.