Isolation and identification of compounds from biofilms in eutrophic water and their allelopathic effects on cyanobacterial growth

Yonghong Wu, Philip Kerr, Yundong Li, Linzhang Yang

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Abstract

That allelochemicals from land plants and macrophytes inhibit cyanobacterial growth has been confirmed. However, there is little information available on allelochemicals from biofilms. This study focused on the extraction, isolation and identification of compounds from photoautotrophic biofilms.Ethyl acetate and ethanol extracted 44 compounds, much more quantity of allelochemicals than dichloromethane, and water did (17 compounds), implying that solvents with polarity index from 4.4 to 5.1 could be suitable extractants for extracting more allelochemicals from biofilms. Total of 22 known and 5 unknown compounds were isolated from the ethyl acetate extract of the biofilms, and 12 known and 5 unknown compounds from methanol, four known and 3 unknown from dichloromethane, and 7 known and 3 unknown from water. The 3,4,5-trihydroxybenzoic acid was the highest amount compounds extracted from biofilms by methanol, which was considered as a potential allelochemical on cyanobacterium because the compound was also an putative allelochemical from land plants and macrophytes.Growth of the cyanobacterium Microcystis aeruginosa was significantly inhibited by 3,4,5-trihydroxybenzoic acid with concentrations from 0.1 mg/L to 0.3 mg/L'and the allelopathic effect increased with time. The known allelopathic compound ethyl-2-methylacetoacetate was also identified. It was concluded therefore that some of the suite of compounds extracted from biofilms were the same as those extracted from land plants or macrophytes. Additionally, currently unidentified compounds extracted from biofilm require bioassay to determine their allelopathic potential for the inhibition of cyanobacterial growth.
Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalAllelopathy Journal
Volume22
Issue number2
Publication statusPublished - 2008

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allelochemicals
biofilm
embryophytes
macrophytes
water
methylene chloride
Cyanobacteria
methanol
Microcystis aeruginosa
acids
bioassays
ethanol
extracts

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title = "Isolation and identification of compounds from biofilms in eutrophic water and their allelopathic effects on cyanobacterial growth",
abstract = "That allelochemicals from land plants and macrophytes inhibit cyanobacterial growth has been confirmed. However, there is little information available on allelochemicals from biofilms. This study focused on the extraction, isolation and identification of compounds from photoautotrophic biofilms.Ethyl acetate and ethanol extracted 44 compounds, much more quantity of allelochemicals than dichloromethane, and water did (17 compounds), implying that solvents with polarity index from 4.4 to 5.1 could be suitable extractants for extracting more allelochemicals from biofilms. Total of 22 known and 5 unknown compounds were isolated from the ethyl acetate extract of the biofilms, and 12 known and 5 unknown compounds from methanol, four known and 3 unknown from dichloromethane, and 7 known and 3 unknown from water. The 3,4,5-trihydroxybenzoic acid was the highest amount compounds extracted from biofilms by methanol, which was considered as a potential allelochemical on cyanobacterium because the compound was also an putative allelochemical from land plants and macrophytes.Growth of the cyanobacterium Microcystis aeruginosa was significantly inhibited by 3,4,5-trihydroxybenzoic acid with concentrations from 0.1 mg/L to 0.3 mg/L'and the allelopathic effect increased with time. The known allelopathic compound ethyl-2-methylacetoacetate was also identified. It was concluded therefore that some of the suite of compounds extracted from biofilms were the same as those extracted from land plants or macrophytes. Additionally, currently unidentified compounds extracted from biofilm require bioassay to determine their allelopathic potential for the inhibition of cyanobacterial growth.",
keywords = "Allelochemicals, Biofilms, Identification, Isolation, Microcystis aeruginosa, Polarity",
author = "Yonghong Wu and Philip Kerr and Yundong Li and Linzhang Yang",
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Isolation and identification of compounds from biofilms in eutrophic water and their allelopathic effects on cyanobacterial growth. / Wu, Yonghong; Kerr, Philip; Li, Yundong; Yang, Linzhang.

In: Allelopathy Journal, Vol. 22, No. 2, 2008, p. 417-428.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and identification of compounds from biofilms in eutrophic water and their allelopathic effects on cyanobacterial growth

AU - Wu, Yonghong

AU - Kerr, Philip

AU - Li, Yundong

AU - Yang, Linzhang

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Allelopathy Journal. ISSNs: 0971-4693;

PY - 2008

Y1 - 2008

N2 - That allelochemicals from land plants and macrophytes inhibit cyanobacterial growth has been confirmed. However, there is little information available on allelochemicals from biofilms. This study focused on the extraction, isolation and identification of compounds from photoautotrophic biofilms.Ethyl acetate and ethanol extracted 44 compounds, much more quantity of allelochemicals than dichloromethane, and water did (17 compounds), implying that solvents with polarity index from 4.4 to 5.1 could be suitable extractants for extracting more allelochemicals from biofilms. Total of 22 known and 5 unknown compounds were isolated from the ethyl acetate extract of the biofilms, and 12 known and 5 unknown compounds from methanol, four known and 3 unknown from dichloromethane, and 7 known and 3 unknown from water. The 3,4,5-trihydroxybenzoic acid was the highest amount compounds extracted from biofilms by methanol, which was considered as a potential allelochemical on cyanobacterium because the compound was also an putative allelochemical from land plants and macrophytes.Growth of the cyanobacterium Microcystis aeruginosa was significantly inhibited by 3,4,5-trihydroxybenzoic acid with concentrations from 0.1 mg/L to 0.3 mg/L'and the allelopathic effect increased with time. The known allelopathic compound ethyl-2-methylacetoacetate was also identified. It was concluded therefore that some of the suite of compounds extracted from biofilms were the same as those extracted from land plants or macrophytes. Additionally, currently unidentified compounds extracted from biofilm require bioassay to determine their allelopathic potential for the inhibition of cyanobacterial growth.

AB - That allelochemicals from land plants and macrophytes inhibit cyanobacterial growth has been confirmed. However, there is little information available on allelochemicals from biofilms. This study focused on the extraction, isolation and identification of compounds from photoautotrophic biofilms.Ethyl acetate and ethanol extracted 44 compounds, much more quantity of allelochemicals than dichloromethane, and water did (17 compounds), implying that solvents with polarity index from 4.4 to 5.1 could be suitable extractants for extracting more allelochemicals from biofilms. Total of 22 known and 5 unknown compounds were isolated from the ethyl acetate extract of the biofilms, and 12 known and 5 unknown compounds from methanol, four known and 3 unknown from dichloromethane, and 7 known and 3 unknown from water. The 3,4,5-trihydroxybenzoic acid was the highest amount compounds extracted from biofilms by methanol, which was considered as a potential allelochemical on cyanobacterium because the compound was also an putative allelochemical from land plants and macrophytes.Growth of the cyanobacterium Microcystis aeruginosa was significantly inhibited by 3,4,5-trihydroxybenzoic acid with concentrations from 0.1 mg/L to 0.3 mg/L'and the allelopathic effect increased with time. The known allelopathic compound ethyl-2-methylacetoacetate was also identified. It was concluded therefore that some of the suite of compounds extracted from biofilms were the same as those extracted from land plants or macrophytes. Additionally, currently unidentified compounds extracted from biofilm require bioassay to determine their allelopathic potential for the inhibition of cyanobacterial growth.

KW - Allelochemicals

KW - Biofilms

KW - Identification

KW - Isolation

KW - Microcystis aeruginosa

KW - Polarity

M3 - Article

VL - 22

SP - 417

EP - 428

JO - Allelopathy Journal

JF - Allelopathy Journal

SN - 0971-4693

IS - 2

ER -