Isolation and partial characterisation of rennet-like proteases from Australian cardoon (Cynara cardunculus L.)

Shaojiang Chen, Jian Zhao, Samson Agboola

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    The yield, protein content, proteolytic activity, and substrate specificity of crude and partially purified extracts from dried and fresh Australian cardoon (Cynara cardunculus L.) flowers were determined. Crude water extracts had high yield but low protein content and proteolytic activity, whereas citric acid extracts had low yield but high protein content and proteolytic activity. Fresh flower extracts gave higher yield and proteolytic activity but lower protein content in comparison with dried flower extracts. Purification with ammonium sulfate resulted in significantly increased proteolytic activity for water extracts from both fresh and dried cardoon flowers, whereas the proteolytic activity of citric acid extracts did not change significantly after purification. Irrespective of extraction method, all extracts had higher proteolytic activity against ovine whole and '-caseins compared to their bovine counterparts, showing optimal activity at 37 °C and pH 6.0. Separation of purified extracts by ion-exchange liquid chromatography yielded three active fractions, each of which when assayed with sodium dodecyl sulfate capillary electrophoresis revealed two subunits with molecular masses of 15.5 and 33.1 kDa, respectively.
    Original languageEnglish
    Pages (from-to)3127-3134
    Number of pages8
    JournalJournal of Agricultural and Food Chemistry
    Volume51
    Issue number10
    DOIs
    Publication statusPublished - 2003

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