Isolation of Toxoplasma gondii from the brain of a dog in Australia and its biological and molecular characterization

Sarwat Al-Qassab, Michael P. Reichel, Chunlei Su, David Jenkins, Craig Hall, Peter A. Windsor, J.P. Dubey, John Ellis

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Toxoplasma gondii was isolated from the brain of a young dog for the first time in Australia. The identity of the parasite was confirmed by PCR, Western blotting, electron microscopy and cat bioassay. Genotyping of the isolate (TgDgAu1) was determined by PCR-RFLP markers that showed it to be a Type II strain. Western blotting demonstrated the presence of IgM antibodies to T. gondii suggesting the bitch was probably infected during pregnancy and the T. gondii was transmitted to the pups congenitally. We believe this represents the first description of a natural case of congenital transmission of T. gondii in the dog.
Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalVeterinary Parasitology
Volume164
Issue number1-2
DOIs
Publication statusPublished - Oct 2009

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