TY - JOUR
T1 - Knockdown of SKA1 gene inhibits cell proliferation and metastasis in human adenoid cystic carcinoma
AU - Zhao, Li juan
AU - Yang, Hong li
AU - Li, Ke yi
AU - Gao, Yue hua
AU - Dong, Kai
AU - Liu, Zhong hao
AU - Wang, Le xin
AU - Zhang, Bin
N1 - Includes bibliographical references.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - The spindle and kinetochore-associated complex subunit 1(SKA1) is a newly discovered gene, which has been associated with mitosis and tumorigenesis. However, its role insalivary adenoid cystic carcinoma (SACC) is still unknown, and the invasive and metastatic mechanism in SACC is still unclear. To explore the molecular mechanism of SKA1 in the process of malignant proliferation and metastasis in adenoid cystic cancer (ACC) cells, we employed lentivirus-mediated short hairpin RNA to knockdown SKA1 in SACC-83 cells. The results demonstrated that the lentivirus-mediated shRNA-targeting SKA1 lead to a significant down-regulation of SKA1 expression. Knockdown of SKA1 inhibited cell proliferation, cell invasion, migration and the cell cycle arrest. Furthermore, knockdown of SKA1 reduced the Ndc80, CDK4, Cyclin D1, Cyclin E1, Cyclin B1 and matrix metalloproteinase-9 (MMP-9) protein expression, but increased the p27 protein expression. These findings indicated that SKA1 might be a promising target for cancer gene therapy in human ACC.
AB - The spindle and kinetochore-associated complex subunit 1(SKA1) is a newly discovered gene, which has been associated with mitosis and tumorigenesis. However, its role insalivary adenoid cystic carcinoma (SACC) is still unknown, and the invasive and metastatic mechanism in SACC is still unclear. To explore the molecular mechanism of SKA1 in the process of malignant proliferation and metastasis in adenoid cystic cancer (ACC) cells, we employed lentivirus-mediated short hairpin RNA to knockdown SKA1 in SACC-83 cells. The results demonstrated that the lentivirus-mediated shRNA-targeting SKA1 lead to a significant down-regulation of SKA1 expression. Knockdown of SKA1 inhibited cell proliferation, cell invasion, migration and the cell cycle arrest. Furthermore, knockdown of SKA1 reduced the Ndc80, CDK4, Cyclin D1, Cyclin E1, Cyclin B1 and matrix metalloproteinase-9 (MMP-9) protein expression, but increased the p27 protein expression. These findings indicated that SKA1 might be a promising target for cancer gene therapy in human ACC.
KW - Metastasis
KW - Proliferation
KW - RNA interference
KW - Salivary adenoid cystic carcinoma (SACC)
KW - Spindle and kinetochore-associated complex subunit 1(SKA1)
KW - Complex subunit 1(SKA1)
UR - http://www.scopus.com/inward/record.url?scp=85015796903&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85015796903&partnerID=8YFLogxK
U2 - 10.1016/j.biopha.2017.03.029
DO - 10.1016/j.biopha.2017.03.029
M3 - Article
C2 - 28340379
AN - SCOPUS:85015796903
VL - 90
SP - 8
EP - 14
JO - Biomedicine
JF - Biomedicine
SN - 0753-3322
ER -
