Laboratory testing for von Willebrand Factor Antigen (VWF:Ag)

Emmanuel J Favaloro, Soma Mohammed, Jürgen Patzke

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

von Willebrand disease (VWD) is reportedly the most common inherited bleeding disorder and can also arise as an acquired syndrome (AVWS). These disorders arise due to defects and/or deficiency of the plasma protein von Willebrand factor (VWF). Laboratory testing for the VWF-related disorders requires assessment of both VWF level and VWF activity, the latter requiring multiple assays because of the many functions carried out by VWF to help prevent bleeding. The current paper describes protocols for assessment of VWF level by means of VWF antigen (VWF:Ag). These assays identify VWF levels by quantitative assessment of VWF protein by means of immunological assays. The most commonly performed assays for VWF:Ag comprise enzyme-linked immunosorbent assays (ELISA) and latex-enhanced immunoassays (LIA), as described in this chapter.

Original languageEnglish
Pages (from-to)403-416
Number of pages14
JournalMethods in Molecular Biology
Volume1646
Early online date14 Aug 2017
DOIs
Publication statusPublished - 2017

Fingerprint

Dive into the research topics of 'Laboratory testing for von Willebrand Factor Antigen (VWF:Ag)'. Together they form a unique fingerprint.

Cite this