Laboratory Testing for von Willebrand Factor Ristocetin Cofactor (VWF:RCo)

Soma Mohammed, Emmanuel J Favaloro

Research output: Book chapter/Published conference paperChapter (peer-reviewed)peer-review

18 Citations (Scopus)


von Willebrand disease (VWD) is reportedly the most common inherited bleeding disorder and can also arise as an acquired syndrome (AVWS). These disorders develop due to defects and/or deficiency of the plasma protein von Willebrand factor (VWF). Laboratory testing for these VWF-related disorders requires assessment of both VWF level and VWF activity, the latter requiring multiple assays because of the many functions carried out by VWF to help prevent bleeding. The current paper describes several protocols for assessment of VWF activity by means of VWF ristocetin cofactor (VWF:RCo). These assays identify VWF activity by quantitative assessment of VWF protein adhesion to platelets or other particles and subsequent detection of the adhered VWF as facilitated by inclusion of ristocetin. The most commonly performed assays for VWF:RCo comprise platelet agglutination assays, latex agglutination assays, and chemiluminescent assay (CLIA), with three of these described in this chapter.

Original languageEnglish
Title of host publicationHemostasis and Thrombosis
Subtitle of host publicationMethods and Protocols
EditorsEmmanuel J. Favaloro, Giuseppe Lippi
Place of PublicationNew York
PublisherHumana Press
Number of pages17
ISBN (Electronic)9781493971961
ISBN (Print)9781493971947
Publication statusPublished - 14 Aug 2017

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


Dive into the research topics of 'Laboratory Testing for von Willebrand Factor Ristocetin Cofactor (VWF:RCo)'. Together they form a unique fingerprint.

Cite this