TY - CHAP
T1 - Laboratory testing protocols for Heparin-Induced Thrombocytopenia (HIT) Testing
AU - Lau, Kun Kan Edwin
AU - Mohammed, Soma
AU - Pasalic, Leonardo
AU - Favaloro, Emmanuel J
PY - 2017/8/14
Y1 - 2017/8/14
N2 - Heparin-induced thrombocytopenia (HIT) represents a significant high morbidity complication of heparin therapy. The clinicopathological diagnosis of HIT remains challenging for many reasons; thus, laboratory testing represents an important component of an accurate diagnosis. Although there are many assays available to assess HIT, these essentially fall into two categories-(a) immunological assays, and (b) functional assays. The current chapter presents protocols for several HIT assays, being those that are most commonly performed in laboratory practice and have the widest geographic distribution. These comprise a manual lateral flow-based system (STiC), a fully automated latex immunoturbidimetric assay, a fully automated chemiluminescent assay (CLIA), light transmission aggregation (LTA), and whole blood aggregation (Multiplate).
AB - Heparin-induced thrombocytopenia (HIT) represents a significant high morbidity complication of heparin therapy. The clinicopathological diagnosis of HIT remains challenging for many reasons; thus, laboratory testing represents an important component of an accurate diagnosis. Although there are many assays available to assess HIT, these essentially fall into two categories-(a) immunological assays, and (b) functional assays. The current chapter presents protocols for several HIT assays, being those that are most commonly performed in laboratory practice and have the widest geographic distribution. These comprise a manual lateral flow-based system (STiC), a fully automated latex immunoturbidimetric assay, a fully automated chemiluminescent assay (CLIA), light transmission aggregation (LTA), and whole blood aggregation (Multiplate).
KW - Anticoagulants/adverse effects
KW - Blood Coagulation Tests/methods
KW - Blood Platelets/drug effects
KW - Heparin/adverse effects
KW - Humans
KW - Immunologic Tests/methods
KW - Immunoturbidimetry/methods
KW - Luminescent Measurements/methods
KW - Platelet Function Tests/methods
KW - Thrombocytopenia/blood
U2 - 10.1007/978-1-4939-7196-1_19
DO - 10.1007/978-1-4939-7196-1_19
M3 - Chapter (peer-reviewed)
C2 - 28804833
SN - 9781493971947
T3 - Methods in Molecular Biology
SP - 227
EP - 243
BT - Hemostasis and Thrombosis
A2 - Favaloro, Emmanuel J.
A2 - Lippi, Giuseppe
CY - New York
ER -