Laccase gene expression during the infection of grape berries by Botrytis cinerea

Aruni Udugama Vithanage, Christopher Steel, Sandra Savocchia, Padraig Strappe, Leigh Schmidtke

Research output: Other contribution to conferenceAbstractpeer-review

Abstract

Laccases produced by Botrytis cinerea during infection of grape bunches can lead to wineoxidation and subsequent detrimental effects on wine quality. The role of laccases in thedevelopment of Botrytis bunch rot has not been fully elucidated even though the researchsuggests the involvement of three genes (LAC1, LAC2, LAC3) in laccase production by B. cinerea.The expression of these genes during infection by B. cinerea on grapes was examined by selectingone botrytis isolate (TN080) following an initial screening of virulence. Sterilized wine grapes(Chardonnay) were inoculated with a 10µl spore suspension (107spores∙ml-1) and incubated at24°C. Berries were collected at different time intervals post-inoculation, frozen in liquid nitrogenand total RNA extracted, followed by cDNA synthesis, RT-qPCR with primers designed for the threeLAC genes. Relative gene expression was normalized to the internal control, actin. Laccase enzymeactivity was also assessed in berry extracts collected at the same time and expressed as U∙ml-1. Allexperiments were done using three biological replicates. The expression of LAC3 was greatest (15fold) at one day post-inoculation and reduced gradually up to day 3. No expression of LAC2 andLAC1 genes were observed. Despite this gene expression, laccase enzyme activity in infected berryextracts was not observed until 3 days post-inoculation. The expression pattern of laccasetranscripts implies the involvement of the LAC3 gene in disease development. LAC3 may also be adeterminant of virulence during the infection of B. cinerea on grapes. This study implies thatlaccases may be important during the early stages of B. cinerea infection on grape berries.
Original languageEnglish
Pages61
Number of pages1
Publication statusPublished - 2016
EventXVII International Botrytis symposium - Santa Cruz Plaza Hotel, Santa Cruz, Chile
Duration: 22 Oct 201627 Oct 2016
https://blueberriesconsulting.com/en/xi-congreso-internacional-vaccinium-2/ (Symposium website)
https://studylib.es/doc/5547636/programme---xvii-international-botrytis-symposium (Program)

Conference

ConferenceXVII International Botrytis symposium
CountryChile
CitySanta Cruz
Period22/10/1627/10/16
OtherThe Botrytis XVII International Symposium will be organized by the Laboratory of Fruity and Molecular Phytopathology of the Department of Plant Health of the Faculty of Agronomic Sciences of the University of Chile, Santiago, Chile.

The Symposium will be held at the Santa Cruz Plaza Hotel (Headquarter Symposium) from the 23 to the 28 October of 2016, in Santa Cruz, a typical city located in the Colchagua Valley, at 180 km (110 miles) south of Santiago .

The symposium, held every three years, offers a very good opportunity to receive updated information on the Botrytis problem and its management in the main crops affected by this pathogen worldwide. The main aspects involved will be: biology, identification, genetics, ecology and epidemiology, post-genomics, interactions of host, cultural pathogens, chemical and biological control and post-harvest management. An overview of the latest results obtained in these aspects of Botrytis will be presented by different teachers.
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