Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers

Leigh Schmidtke, Jeremy Carson

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5 Citations (Scopus)

Abstract

Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.
Original languageEnglish
Pages (from-to)206-212
Number of pages7
JournalBulletin of the European Association of Fish Pathologists
Volume23
Issue number5
Publication statusPublished - 2003

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Lactococcus
Lactococcus garvieae
repetitive sequences
Nucleic Acid Repetitive Sequences
Oncorhynchus mykiss
South Africa
Tasmania
rainbow
Japan
pathogen
Victoria
Polymerase Chain Reaction
Victoria (Australia)
targeting
South Australia
DNA Primers
Zoonoses
DNA
Seriola quinqueradiata
Fishes

Cite this

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title = "Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers",
abstract = "Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.",
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TY - JOUR

T1 - Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers

AU - Schmidtke, Leigh

AU - Carson, Jeremy

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = European Association of Fish Pathologists. Bulletin. ISSNs: 0108-0288;

PY - 2003

Y1 - 2003

N2 - Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.

AB - Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.

KW - ENTEROCOCCUS-SERIOLICIDA

KW - FISH

KW - PCR

KW - PRIMERS

KW - SYNONYM

M3 - Article

VL - 23

SP - 206

EP - 212

JO - Bulletin of the European Association of Fish Pathologists

JF - Bulletin of the European Association of Fish Pathologists

SN - 0108-0288

IS - 5

ER -