Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers

Leigh Schmidtke, Jeremy Carson

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    5 Citations (Scopus)

    Abstract

    Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.
    Original languageEnglish
    Pages (from-to)206-212
    Number of pages7
    JournalBulletin of the European Association of Fish Pathologists
    Volume23
    Issue number5
    Publication statusPublished - 2003

    Fingerprint

    Lactococcus
    Lactococcus garvieae
    repetitive sequences
    Nucleic Acid Repetitive Sequences
    Oncorhynchus mykiss
    South Africa
    Tasmania
    rainbow
    Japan
    pathogen
    Victoria
    Polymerase Chain Reaction
    Victoria (Australia)
    targeting
    South Australia
    DNA Primers
    Zoonoses
    DNA
    Seriola quinqueradiata
    Fishes

    Cite this

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    title = "Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers",
    abstract = "Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.",
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    T1 - Lactococcus garvieae strains isolated from rainbow trout and yellowtail in Australia, South Africa and Japan differentiated by repetitive sequence markers

    AU - Schmidtke, Leigh

    AU - Carson, Jeremy

    N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = European Association of Fish Pathologists. Bulletin. ISSNs: 0108-0288;

    PY - 2003

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    N2 - Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.

    AB - Lactococcus garvieae is an emerging important epizootiological pathogen. A total of 25 Lactococcus garvieae isolates from diseased rainbow trout (Oncorhynchus mykiss) and yellowtail (Seriola quinqueradiata) from Japan, South Africa and Australia (Tasmania and Victoria) and type strains were analysed by repetitive sequence based PCR targeting repetitive extragenic palindromes (REP) and BOX interspersed DNA sequences. Both primer sets generated five different banding profiles with isolates from South Africa, Tasmania and Victoria sharing common profiles. Japanese isolates could easily be distinguished from all other isolates on the basis of banding profile and BOX primers enabled differentiation of capsular types for these strains. The type strain L. garvieae NCFB 2157 produced a unique profile significantly different from other strains with both oligonucleotide primer sets. REP-PCR and BOX-PCR offer a rapid, cost effective and straightforward means for strain differentiation of this important fish and emerging zoonotic pathogen.

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    KW - PCR

    KW - PRIMERS

    KW - SYNONYM

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    JO - Bulletin of the European Association of Fish Pathologists

    JF - Bulletin of the European Association of Fish Pathologists

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