TY - JOUR
T1 - Laser induced self-N-doped porous graphene as an electrochemical biosensor for femtomolar miRNA detection
AU - Wan, Zhengfen
AU - Umer, Muhammad
AU - Lobino, Mirko
AU - Thiel, David
AU - Nguyen, Nam Trung
AU - Trinchi, Adrian
AU - Shiddiky, Muhammad J.A.
AU - Gao, Yongsheng
AU - Li, Qin
N1 - Funding Information:
Z. Wan thanks to the support of the Griffith University International Postgraduate Scholarship and a CSIRO Top-up Scholarship. The authors acknowledge the funding support of Australian Research Council Industry Transformational Research Hub ( IH 180100002 ). This work was performed in part at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australia’s researchers. M. Lobino was supported by the Australian Research Council (ARC) Future Fellowship ( FT180100055 ). The authors also thank the technical support from the Queensland Micro- and Nanotechnology Centre at Griffith University, and the Centre for Microscopy and Microanalysis at the University of Queensland.
Funding Information:
Z. Wan thanks to the support of the Griffith University International Postgraduate Scholarship and a CSIRO Top-up Scholarship. The authors acknowledge the funding support of Australian Research Council Industry Transformational Research Hub (IH 180100002). This work was performed in part at the Queensland node of the Australian National Fabrication Facility, a company established under the National Collaborative Research Infrastructure Strategy to provide nano- and micro-fabrication facilities for Australia's researchers. M. Lobino was supported by the Australian Research Council (ARC) Future Fellowship (FT180100055). The authors also thank the technical support from the Queensland Micro- and Nanotechnology Centre at Griffith University, and the Centre for Microscopy and Microanalysis at the University of Queensland.
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/8/15
Y1 - 2020/8/15
N2 - We report a sensitive, yet low-cost biosensor based on laser induced graphene for femtomolar microRNA (miRNA) detection. Combined with the miRNA extraction and magnetic isolation process, the target miRNAs were purified for further detection using laser induced graphene sensor. The laser induced graphene was prepared by direct laser writing on commercial polyimide (PI) and patterned via a computer-aided design system as an electrode for electrochemical biosensing. We found that the laser reduction of PI resulted in nitrogen-doped porous graphene, not only improving its conductivity but also its sensitivity to nucleic acids. Preeclampsia specific miRNA hsa-miR-486-5p was magnetically purified and directly adsorbed on the surface of graphene electrode via graphene-miRNA affinity interaction. Surface attached miRNAs were then electrochemically quantified using [Fe(CN)6]3-/4- redox system. Our assay demonstrates detection of miRNA has-miR-486-5p up to concentrations as low as 10 fM with excellent reproducibility. Owing to its facile fabrication, low cost and high performance, the laser induced N-doped graphene biosensor presented here shows great potential for applications in detecting miRNA in biomedical applications.
AB - We report a sensitive, yet low-cost biosensor based on laser induced graphene for femtomolar microRNA (miRNA) detection. Combined with the miRNA extraction and magnetic isolation process, the target miRNAs were purified for further detection using laser induced graphene sensor. The laser induced graphene was prepared by direct laser writing on commercial polyimide (PI) and patterned via a computer-aided design system as an electrode for electrochemical biosensing. We found that the laser reduction of PI resulted in nitrogen-doped porous graphene, not only improving its conductivity but also its sensitivity to nucleic acids. Preeclampsia specific miRNA hsa-miR-486-5p was magnetically purified and directly adsorbed on the surface of graphene electrode via graphene-miRNA affinity interaction. Surface attached miRNAs were then electrochemically quantified using [Fe(CN)6]3-/4- redox system. Our assay demonstrates detection of miRNA has-miR-486-5p up to concentrations as low as 10 fM with excellent reproducibility. Owing to its facile fabrication, low cost and high performance, the laser induced N-doped graphene biosensor presented here shows great potential for applications in detecting miRNA in biomedical applications.
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U2 - 10.1016/j.carbon.2020.03.043
DO - 10.1016/j.carbon.2020.03.043
M3 - Article
AN - SCOPUS:85082654327
SN - 0008-6223
VL - 163
SP - 385
EP - 394
JO - Carbon
JF - Carbon
ER -