Liver fluke vaccines: Vaccination Against Fasciolosis by a Multivalent Vaccine of Recombinant Stage-Specific Antigens

R. Jayaraj, D. Piedrafita, K. Dynon, R. Grams, Terry Spithill, P.M. Smooker

Research output: Book chapter/Published conference paperConference paper

2 Citations (Scopus)
1 Downloads (Pure)

Abstract

Fasciola's excretory-secretory material comprises chiefly cathepsin B and cathepsin L. These cysteine proteases are proposed as major mediators of parasitism, and are considered targets for vaccination. In order to assess the vaccine efficacy of these enzymes, single and multivalent recombinant protein vaccinations of adult-stage F. hepatica cathepsin L5, metacercarial-stage F. gigantica cathepsin L1 g and juvenile-stage F. hepatica cathepsin B were analysed in rats against F. hepatica challenge infection. The protective efficacy of anti-fluke vaccines was evaluated in terms of parasitological parameters (recovered fluke burden, fluke body size and wet weight) and pathological changes (liver damage score) in rats. The rats vaccinated with recombinant proteins were shown to have significantly fewer and smaller flukes than the control rats. A maximum protection of 83% was seen in the group vaccinated with a combination of cathepsin B and cathepsin L5.
Original languageEnglish
Title of host publication3rd proceedings
Place of PublicationNetherlands
PublisherElsevier BV
Pages82-85
Number of pages4
Volume2
DOIs
Publication statusPublished - 2010
EventGlobal Vaccine Congress - Singapore, Singapore
Duration: 04 Oct 200906 Oct 2009

Conference

ConferenceGlobal Vaccine Congress
CountrySingapore
Period04/10/0906/10/09

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    Jayaraj, R., Piedrafita, D., Dynon, K., Grams, R., Spithill, T., & Smooker, P. M. (2010). Liver fluke vaccines: Vaccination Against Fasciolosis by a Multivalent Vaccine of Recombinant Stage-Specific Antigens. In 3rd proceedings (Vol. 2, pp. 82-85). Elsevier BV. https://doi.org/10.1016/j.provac.2010.03.015