Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs

Maria Karayannopoulou, Lysimachos Papazoglou, Panayiotis Loukopoulos, G. Kazakos, A. Chantes, N. Giannakas, I. Savvas, Dimitra Psalla, Maria Kritsepi-Konstantinou, D. Dionyssiou

Research output: Other contribution to conferenceAbstract

12 Citations (Scopus)

Abstract

Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs. Methods: A 2x10 cm subdermal plexus skin flap was created bilaterally on the abdominal wall of six Beagle dogs. One randomly selected side received 2.5 ml of fresh autologous PRP injected evenly between sutures underneath the flap, whereas the other side was left untreated (control). Skin flap survival was evaluated macroscopically, histologically and by laser-Doppler flowmetry measurements of tissue perfusion. Results: Flap percentage survival on day 10 (96.3% versus 74.5%; p = 0.046) and tissue perfusion (p <0.036) were significantly higher in PRP-treated flaps compared with controls. Histologically, there was less oedema in PRP-treated flaps compared to controls (p = 0.01), whereas collagen production and angiogenesis did not differ significantly between the two groups. Clinical significance: The use of locally injected autologous PRP increases tissue perfusion and improves the survival of long subdermal plexus skin flaps in dogs.
Original languageEnglish
Pages379-386
Number of pages8
Publication statusPublished - 2014
Event10th Panhellenic Veterinary Congress of Companion Animals - Divani Palace Acropolis Hotel, Athens, Greece
Duration: 01 Nov 201303 Nov 2013
http://athenscvb.gr/en/content/10th-panhellenic-veterinary-congress-companion-animals

Conference

Conference10th Panhellenic Veterinary Congress of Companion Animals
Abbreviated titlePediatrics and Geriatrics
CountryGreece
CityAthens
Period01/11/1303/11/13
Internet address

Fingerprint

Platelet-Rich Plasma
Perfusion
Dogs
Skin
Laser-Doppler Flowmetry
Abdominal Wall
Sutures
Edema
Necrosis
Collagen

Cite this

Karayannopoulou, M., Papazoglou, L., Loukopoulos, P., Kazakos, G., Chantes, A., Giannakas, N., ... Dionyssiou , D. (2014). Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. 379-386. Abstract from 10th Panhellenic Veterinary Congress of Companion Animals, Athens, Greece.
Karayannopoulou, Maria ; Papazoglou, Lysimachos ; Loukopoulos, Panayiotis ; Kazakos, G. ; Chantes, A. ; Giannakas, N. ; Savvas, I. ; Psalla, Dimitra ; Kritsepi-Konstantinou, Maria ; Dionyssiou , D. / Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. Abstract from 10th Panhellenic Veterinary Congress of Companion Animals, Athens, Greece.8 p.
@conference{3f9a3057a57f4bc5b417fabf01e4d724,
title = "Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs",
abstract = "Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs. Methods: A 2x10 cm subdermal plexus skin flap was created bilaterally on the abdominal wall of six Beagle dogs. One randomly selected side received 2.5 ml of fresh autologous PRP injected evenly between sutures underneath the flap, whereas the other side was left untreated (control). Skin flap survival was evaluated macroscopically, histologically and by laser-Doppler flowmetry measurements of tissue perfusion. Results: Flap percentage survival on day 10 (96.3{\%} versus 74.5{\%}; p = 0.046) and tissue perfusion (p <0.036) were significantly higher in PRP-treated flaps compared with controls. Histologically, there was less oedema in PRP-treated flaps compared to controls (p = 0.01), whereas collagen production and angiogenesis did not differ significantly between the two groups. Clinical significance: The use of locally injected autologous PRP increases tissue perfusion and improves the survival of long subdermal plexus skin flaps in dogs.",
keywords = "Wound healing, dog, pathology, Comparative pathology, veterinary surgery",
author = "Maria Karayannopoulou and Lysimachos Papazoglou and Panayiotis Loukopoulos and G. Kazakos and A. Chantes and N. Giannakas and I. Savvas and Dimitra Psalla and Maria Kritsepi-Konstantinou and D. Dionyssiou",
note = "Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Veterinary and Comparative Orthopaedics and Traumatology. ISSNs: 0932-0814; ; 10th Panhellenic Veterinary Congress of Companion Animals, Pediatrics and Geriatrics ; Conference date: 01-11-2013 Through 03-11-2013",
year = "2014",
language = "English",
pages = "379--386",
url = "http://athenscvb.gr/en/content/10th-panhellenic-veterinary-congress-companion-animals",

}

Karayannopoulou, M, Papazoglou, L, Loukopoulos, P, Kazakos, G, Chantes, A, Giannakas, N, Savvas, I, Psalla, D, Kritsepi-Konstantinou, M & Dionyssiou , D 2014, 'Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs' 10th Panhellenic Veterinary Congress of Companion Animals, Athens, Greece, 01/11/13 - 03/11/13, pp. 379-386.

Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. / Karayannopoulou, Maria; Papazoglou, Lysimachos; Loukopoulos, Panayiotis; Kazakos, G.; Chantes, A.; Giannakas, N.; Savvas, I.; Psalla, Dimitra; Kritsepi-Konstantinou, Maria; Dionyssiou , D.

2014. 379-386 Abstract from 10th Panhellenic Veterinary Congress of Companion Animals, Athens, Greece.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs

AU - Karayannopoulou, Maria

AU - Papazoglou, Lysimachos

AU - Loukopoulos, Panayiotis

AU - Kazakos, G.

AU - Chantes, A.

AU - Giannakas, N.

AU - Savvas, I.

AU - Psalla, Dimitra

AU - Kritsepi-Konstantinou, Maria

AU - Dionyssiou , D.

N1 - Imported on 12 Apr 2017 - DigiTool details were: Journal title (773t) = Veterinary and Comparative Orthopaedics and Traumatology. ISSNs: 0932-0814;

PY - 2014

Y1 - 2014

N2 - Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs. Methods: A 2x10 cm subdermal plexus skin flap was created bilaterally on the abdominal wall of six Beagle dogs. One randomly selected side received 2.5 ml of fresh autologous PRP injected evenly between sutures underneath the flap, whereas the other side was left untreated (control). Skin flap survival was evaluated macroscopically, histologically and by laser-Doppler flowmetry measurements of tissue perfusion. Results: Flap percentage survival on day 10 (96.3% versus 74.5%; p = 0.046) and tissue perfusion (p <0.036) were significantly higher in PRP-treated flaps compared with controls. Histologically, there was less oedema in PRP-treated flaps compared to controls (p = 0.01), whereas collagen production and angiogenesis did not differ significantly between the two groups. Clinical significance: The use of locally injected autologous PRP increases tissue perfusion and improves the survival of long subdermal plexus skin flaps in dogs.

AB - Objectives: Distal flap necrosis remains a major complication in subdermal plexus (random) skin flaps. Platelet-rich plasma (PRP) has been shown to improve the survival of ischemic random skin flaps in rats. The objective of this study was to evaluate the effect of locally injected autologous PRP on the survival of long (5:1 length-to-width ratio) subdermal plexus skin flaps in dogs. Methods: A 2x10 cm subdermal plexus skin flap was created bilaterally on the abdominal wall of six Beagle dogs. One randomly selected side received 2.5 ml of fresh autologous PRP injected evenly between sutures underneath the flap, whereas the other side was left untreated (control). Skin flap survival was evaluated macroscopically, histologically and by laser-Doppler flowmetry measurements of tissue perfusion. Results: Flap percentage survival on day 10 (96.3% versus 74.5%; p = 0.046) and tissue perfusion (p <0.036) were significantly higher in PRP-treated flaps compared with controls. Histologically, there was less oedema in PRP-treated flaps compared to controls (p = 0.01), whereas collagen production and angiogenesis did not differ significantly between the two groups. Clinical significance: The use of locally injected autologous PRP increases tissue perfusion and improves the survival of long subdermal plexus skin flaps in dogs.

KW - Wound healing, dog, pathology

KW - Comparative pathology

KW - veterinary surgery

UR - https://www.ncbi.nlm.nih.gov/pubmed/25088504

M3 - Abstract

SP - 379

EP - 386

ER -

Karayannopoulou M, Papazoglou L, Loukopoulos P, Kazakos G, Chantes A, Giannakas N et al. Locally injected autologous platelet-rich plasma enhanced tissue perfusion and improved survival of long subdermal plexus skin flaps in dogs. 2014. Abstract from 10th Panhellenic Veterinary Congress of Companion Animals, Athens, Greece.