Maintaining the fertility of bull spermatozoa during room temperature storage

Erin Kathleen Klein, Allan Gunn, Cyril Stephen, Aleona Swegen, Robert Aitken, Zamira Gibb

Research output: Contribution to journalArticlepeer-review


Traditional sperm storage methods involve cryopreservation or chilling to slow the sperm metabolism and maintain fertility for an extended period of time. In order to store spermatozoa at room temperature (RT), the uninhibited sperm metabolism needs to be supported by the storage medium. We hypothesised that the replacement of NaCl in Biggers, Whitten, and Whittingham (BWW) medium, with alternative osmolytes would lessen the depletion of ATP due to maintenance of Na+home-ostasis. Semen was collected by electroejaculation and good-quality spermatozoa were isolated using density gradient centrifugation.
Original languageEnglish
Article number106369
Pages (from-to)5-6
Number of pages2
JournalAnimal Production Science
Publication statusPublished - Sep 2020


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