Current work health and safety practices focus predominately on fostering a safety climate to promote safety behaviours and reduce workplace accidents. Despite the importance of safety climates in accident prevention, recent research has demonstrated that individual factors can also predict work safety behaviour. This study considered the importance of organisational climate together with individual characteristics including differences in personality, impulsiveness, and perceptions of safety within the workplace on safety behaviour. 203 participants consisting of 67 males and 136 females aged 18 to 71 years, completed an online questionnaire. Results revealed that safety behaviour was directly related to safety climate, and conscientiousness. In contrast, neuroticism, and impulsiveness were not significantly related to safety behaviour. The present study findings support previous findings in the literature regarding the importance of safety climate as well as the personality trait of conscientiousness in applying safety behaviours. However, the present study findings did not support previous research in relation to the personality trait of high neuroticism resulting in decreased safety behaviour, nor did not confirm an inverse relationship between high impulsivity and low safety behaviour as theoretical models would suggest. This new finding may warrant further research into the precursors for safety behaviour.