Matrine displayed antiviral activity in porcine alveolar macrophages co-infected by porcine reproductive and respiratory syndrome virus and porcine circovirus type 2

Na Sun, Panpan Sun, Haipeng Lv, Yaogui Sun, Jianhua Guo, Zhirui Wang, Tiantian Luo, Shaoyu Wang, Hongquan Li

Research output: Contribution to journalArticle

38 Citations (Scopus)
6 Downloads (Pure)

Abstract

The co-infection of porcine reproductive respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) is quite common in clinical settings and no effective treatment to the co-infection is available. In this study, we established the porcine alveolar macrophages (PAM) cells model co-infected with PRRSV/PCV2 with modification in vitro, and investigated the antiviral activity of Matrine on this cell model and further evaluated the effect of Matrine on virus-induced TLR3,4/NF-κ/TNF-α pathway. The results demonstrated PAM cells inoculated with PRRSV followed by PCV2 2 h later enhanced PRRSV and PCV2 replications. Matrine treatment suppressed both PRRSV and PCV2 infection at 12 h post infection. Furthermore, PRRSV/PCV2 co-infection induced Iκα degradation and phosphorylation as well as the translocation of NF-κ from the cytoplasm to the nucleus indicating that PRRSV/PCV2 co-infection induced NF-κ activation. Matrine treatment significantly down-regulated the expression of TLR3, TLR4 and TNF-α although it, to some extent, suppressed p-Iκα expression, suggesting that TLR3,4/NF-κ/TNF-α pathway play an important role of Matrine in combating PRRSV/PCV2 co-infection. It is concluded that Matrine possesses activity against PRRSV/PCV2 co-infection in vitro and suppression of the TLR3,4/NF-κ/TNF-α pathway as an important underlying molecular mechanism. These findings warrant Matrine to be further explored for its antiviral activity in clinical settings.
Original languageEnglish
Article number24401
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume6
Issue number24401
DOIs
Publication statusPublished - 2016

Fingerprint Dive into the research topics of 'Matrine displayed antiviral activity in porcine alveolar macrophages co-infected by porcine reproductive and respiratory syndrome virus and porcine circovirus type 2'. Together they form a unique fingerprint.

  • Cite this