Measurement of antioxidant activity with the thiobarbituric acid reactive substances assay

Md Ahsan Ghani; Celia Barril; Danny R. Bedgood; Paul D. Prenzler

doi:10.1016/j.foodchem.2017.02.127

Measurement of antioxidant activity with the thiobarbituric acid reactive substances assay

Md Ahsan Ghani, Celia Barril, Danny R. Bedgood, Paul D. Prenzler

Research output: Contribution to journal › Article › peer-review

198 Citations (Scopus)

Abstract

The thiobarbituric acid reactive substances (TBARS) assay is widely used to measure lipid oxidation and antioxidant activity in food and physiological systems. However, there has been no review (to our knowledge) that focuses exclusively on this test. This review presents an overview of the current use of the TBARS test in food and physiological systems, before looking at the various ways in which the assay is used in studies on antioxidant activity. As an antioxidant assay, the TBARS test may lack acceptable reproducibility, and long reaction times may preclude its adoption as a rapid screening method. Despite these potential limitations, there are features of the TBARS test that make it useful as a complement to popular screening tests such as Trolox equivalent antioxidant capacity. This review concludes with proposals for development of the TBARS test so that it can be used as a rapid and robust antioxidant assay.

Original language	English
Pages (from-to)	195-207
Number of pages	13
Journal	Food Chemistry
Volume	230
Early online date	Feb 2017
DOIs	https://doi.org/10.1016/j.foodchem.2017.02.127
Publication status	Published - 01 Sept 2017

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10.1016/j.foodchem.2017.02.127

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title = "Measurement of antioxidant activity with the thiobarbituric acid reactive substances assay",

abstract = "The thiobarbituric acid reactive substances (TBARS) assay is widely used to measure lipid oxidation and antioxidant activity in food and physiological systems. However, there has been no review (to our knowledge) that focuses exclusively on this test. This review presents an overview of the current use of the TBARS test in food and physiological systems, before looking at the various ways in which the assay is used in studies on antioxidant activity. As an antioxidant assay, the TBARS test may lack acceptable reproducibility, and long reaction times may preclude its adoption as a rapid screening method. Despite these potential limitations, there are features of the TBARS test that make it useful as a complement to popular screening tests such as Trolox equivalent antioxidant capacity. This review concludes with proposals for development of the TBARS test so that it can be used as a rapid and robust antioxidant assay.",

keywords = "Antioxidant activity, Lipid substrate, Methodology, Screening, TBARS assay",

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AU - Ghani, Md Ahsan

AU - Barril, Celia

AU - Bedgood, Danny R.

AU - Prenzler, Paul D.

N1 - Includes bibliographical references.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - The thiobarbituric acid reactive substances (TBARS) assay is widely used to measure lipid oxidation and antioxidant activity in food and physiological systems. However, there has been no review (to our knowledge) that focuses exclusively on this test. This review presents an overview of the current use of the TBARS test in food and physiological systems, before looking at the various ways in which the assay is used in studies on antioxidant activity. As an antioxidant assay, the TBARS test may lack acceptable reproducibility, and long reaction times may preclude its adoption as a rapid screening method. Despite these potential limitations, there are features of the TBARS test that make it useful as a complement to popular screening tests such as Trolox equivalent antioxidant capacity. This review concludes with proposals for development of the TBARS test so that it can be used as a rapid and robust antioxidant assay.

AB - The thiobarbituric acid reactive substances (TBARS) assay is widely used to measure lipid oxidation and antioxidant activity in food and physiological systems. However, there has been no review (to our knowledge) that focuses exclusively on this test. This review presents an overview of the current use of the TBARS test in food and physiological systems, before looking at the various ways in which the assay is used in studies on antioxidant activity. As an antioxidant assay, the TBARS test may lack acceptable reproducibility, and long reaction times may preclude its adoption as a rapid screening method. Despite these potential limitations, there are features of the TBARS test that make it useful as a complement to popular screening tests such as Trolox equivalent antioxidant capacity. This review concludes with proposals for development of the TBARS test so that it can be used as a rapid and robust antioxidant assay.

KW - Antioxidant activity

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KW - Methodology

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KW - TBARS assay

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Measurement of antioxidant activity with the thiobarbituric acid reactive substances assay

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