Mediated xanthine oxidase potentiometric biosensors for hypoxanthine based on ferrocene carboxylic acid modified electrode

Abdulazeez T. Lawal, Samuel B. Adeloju

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05 M phosphate buffer (pH 7.1) at 0.0 mV vs Ag/AgCl (3 M KCl). The optimum condition for the formation of PPy-XOD-Fc film include 0.4 M PPy, 6.2 U/mL XOD, 40 mM Fc, polymerisation time of 200 s and applied current density of 0-5 mA cm-2. The sensor provides a linear response to Hx in concentration range of 5-20 μM, (r = 0.998) and was successfully used for determination of Hx in fish.

Original languageEnglish
Pages (from-to)2982-2987
Number of pages6
JournalFood Chemistry
Volume135
Issue number4
DOIs
Publication statusPublished - 15 Dec 2012

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hypoxanthine
Hypoxanthine
xanthine oxidase
Xanthine Oxidase
biosensors
Biosensing Techniques
carboxylic acids
Biosensors
electrodes
Electrodes
Polymerization
polymerization
Fish
sensors (equipment)
Fishes
Enzyme electrodes
fish meat
Meats
Sensors
Meat

Cite this

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title = "Mediated xanthine oxidase potentiometric biosensors for hypoxanthine based on ferrocene carboxylic acid modified electrode",
abstract = "A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05 M phosphate buffer (pH 7.1) at 0.0 mV vs Ag/AgCl (3 M KCl). The optimum condition for the formation of PPy-XOD-Fc film include 0.4 M PPy, 6.2 U/mL XOD, 40 mM Fc, polymerisation time of 200 s and applied current density of 0-5 mA cm-2. The sensor provides a linear response to Hx in concentration range of 5-20 μM, (r = 0.998) and was successfully used for determination of Hx in fish.",
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Mediated xanthine oxidase potentiometric biosensors for hypoxanthine based on ferrocene carboxylic acid modified electrode. / Lawal, Abdulazeez T.; Adeloju, Samuel B.

In: Food Chemistry, Vol. 135, No. 4, 15.12.2012, p. 2982-2987.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Mediated xanthine oxidase potentiometric biosensors for hypoxanthine based on ferrocene carboxylic acid modified electrode

AU - Lawal, Abdulazeez T.

AU - Adeloju, Samuel B.

PY - 2012/12/15

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N2 - A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05 M phosphate buffer (pH 7.1) at 0.0 mV vs Ag/AgCl (3 M KCl). The optimum condition for the formation of PPy-XOD-Fc film include 0.4 M PPy, 6.2 U/mL XOD, 40 mM Fc, polymerisation time of 200 s and applied current density of 0-5 mA cm-2. The sensor provides a linear response to Hx in concentration range of 5-20 μM, (r = 0.998) and was successfully used for determination of Hx in fish.

AB - A potentiometric enzyme electrode for detection of hypoxanthine (Hx) in fish meat is described. The sensor was developed by entrapment of xanthine oxidase (XOD) and ferrocene carboxylic acid (Fc) into polypyrrole (PPy) film during galvanostatic polymerisation film formation. The responses for Hx were obtained in 0-05 M phosphate buffer (pH 7.1) at 0.0 mV vs Ag/AgCl (3 M KCl). The optimum condition for the formation of PPy-XOD-Fc film include 0.4 M PPy, 6.2 U/mL XOD, 40 mM Fc, polymerisation time of 200 s and applied current density of 0-5 mA cm-2. The sensor provides a linear response to Hx in concentration range of 5-20 μM, (r = 0.998) and was successfully used for determination of Hx in fish.

KW - Biosensor

KW - Fish

KW - Hypoxanthine

KW - Immobilisation

KW - Oxidase

KW - Potentiometry

KW - Xanthine

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