Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes

James M Mwendwa, Paul A. Weston, Inge Fomsgaard, Bente B Laursen, William B. Brown, Hanwen Wu, Greg Rebetzke, Jane C. Quinn, Leslie A. Weston

    Research output: Book chapter/Published conference paperConference paperpeer-review

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    Replicated wheat (Triticum aestivum L.) cultivar trials were performed in commercial fields in moderate to low rainfall zones at Wagga Wagga (572 mm) and Condobolin (449 mm) NSW in 2014 and 2015. At each experimental site, crop and/or weed growth were monitored at selected growth stages including tillering, vegetative, grain filling, harvest and post-harvest to the crop. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected. All shoot and root samples were extracted in methanol using an automated Buchi high pressure extractor while soil samples were extracted using a rotary shaking method. Extracts were profiled for unique secondary plant products including benzoxazinoids (BXs) using liquid chromatography coupled to mass spectrometry.
    Metabolic profiling of wheat cultivar shoots, roots, and soils resulted in detection of up to 14 BXs including BX glycosides and other metabolites of interest. Both qualitative and quantitative differences in BXs were observed and were cultivar and location dependent. Plant part and rhizosphere location (distance from root) also impacted BX concentration. Additional metabolic profiling is now underway and will provide crucial information regarding crop metabolism and biosynthesis of metabolites associated with weed suppression in commercial wheat cultivars.
    Original languageEnglish
    Title of host publicationProceedings of the 20th Australasian weeds conference
    EditorsRod Randall, Sandy Lloyd, Catherine Borger
    Place of PublicationPerth
    PublisherWeed Society of Western Australia
    Number of pages5
    ISBN (Electronic)9780646960319
    Publication statusPublished - 2016
    Event20th Australasian Weeds Conference (2016) - Pan Pacific Hotel, Perth, Australia
    Duration: 11 Sept 201615 Sept 2016 (Conference website) (Conference proceedings)


    Conference20th Australasian Weeds Conference (2016)
    Abbreviated titleScience, Community and Food Security: the Weed Challenge
    OtherIn 2016, the Council of Australasian Weed Societies (CAWS), along with local hosts the Weeds Society of Western Australia, are celebrating the 20th Australasian Weeds Conference. The biennial conference is being held at the Pan Pacific in Perth, Western Australia which has traditionally been one of the highest attended AWC's, and over 300 delegates are expected from across Australasia.
    Delegates will come together to meet-up with peers, engage with industry sponsors, and to listen and participate in presentations and field trips on a variety of topics including:

    Herbicide Resistance
    Weeds of National Significance
    Agricultural weeds
    Environmental weeds
    Biological control of weeds
    Modelling and new technologies to manage weeds
    Legislation, regulation and policy to manage weed
    Internet address

    Grant Number

    • GRDC UCS 00020
    • GRDC UCS00022
    • GRDC UCS 00023


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