Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes

James M Mwendwa, Paul Weston, Inge Fomsgaard, Bente B Laursen, William Brown, Hanwen Wu, Jane Quinn, Jeffrey Weidenhamer, Razia Shaik, Leslie Weston

Research output: Other contribution to conferenceAbstract

Abstract

Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted in commercial fields in moderate to low rainfall zones at Wagga Wagga (572 mm average) and Condobolin (449 mm average) NSW, respectively in 2014, 2015 and 2016. At each experimental site, crop and/or weed growth were monitored at selected growth stages including tillering, vegetative, grain filling, harvest and after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected. All shoot and root samples were extracted in methanol using an automated Buchi high pressure extractor while soil samples were extracted using a rotary shaking method. Extracts were profiled for unique secondary plant products acting as allelochemicals for weed suppression, specifically benzoxazinoids (BXs) (Fomsgaard et al. 2006), using liquid chromatograph coupled to a triple quadrupole mass spectrometer (UPLC-MS QQQ). In addition, non-targeted metabolomics analysis was performed to evaluate relative abundance of diverse metabolites using a quadrupole time-of-flight mass spectrometry (UPLC-MS QToF) platform. Metabolic profiling of wheat shoots, roots, and soils resulted in detection of up to 14 BXs including BX glycosides and other metabolites of interest (Adhikari et al. Tanwir et al. 2013). Both qualitative and quantitative differences in BXs were observed and were cultivar-, growth stage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BX concentration. The distribution of the secondary metabolites in wheat cultivar tissues suggest differential production of some key bioactive metabolites. Further metabolic profiling provided crucial information regarding crop metabolism, as well as the biosynthesis and release of metabolites associated with weed suppression in currently available commercial wheat cultivars, in contrast to weed suppressive rye (Secale cereale L.) and heritage wheat cultivars such as Federation, known for their potent ability to suppress weeds. This presentation will focus on the results of three years of field experimentation at two locations and predict which cultivars are best-suited for weed suppressive properties due to canopy architecture and allelopathic traits while maintaining high yield potential.
Original languageEnglish
Publication statusPublished - 2017
Event8th World Congress of Allelopathy: IAS - Villa Mediterranee, Marseille, France
Duration: 24 Jul 201728 Jul 2017
https://wca2017.sciencesconf.org/

Conference

Conference8th World Congress of Allelopathy
Abbreviated titleAlellopathy for sustainable ecosystems
CountryFrance
CityMarseille
Period24/07/1728/07/17
Internet address

Fingerprint

benzoxazinoids
metabolomics
vigor
weeds
wheat
genotype
cultivars
metabolites
shoots
weed control
rhizosphere
crops
soil sampling
developmental stages
rhizoplane
extractors
plant products
allelochemicals
Secale cereale
tillering

Grant Number

  • GRDC UCS 00020
  • GRDC UCS 00022
  • GRDC UCS 00023

Cite this

Mwendwa, J. M., Weston, P., Fomsgaard, I., Laursen, B. B., Brown, W., Wu, H., ... Weston, L. (2017). Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes. Abstract from 8th World Congress of Allelopathy, Marseille, France.
Mwendwa, James M ; Weston, Paul ; Fomsgaard, Inge ; Laursen, Bente B ; Brown, William ; Wu, Hanwen ; Quinn, Jane ; Weidenhamer, Jeffrey ; Shaik, Razia ; Weston, Leslie. / Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes. Abstract from 8th World Congress of Allelopathy, Marseille, France.
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abstract = "Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted in commercial fields in moderate to low rainfall zones at Wagga Wagga (572 mm average) and Condobolin (449 mm average) NSW, respectively in 2014, 2015 and 2016. At each experimental site, crop and/or weed growth were monitored at selected growth stages including tillering, vegetative, grain filling, harvest and after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected. All shoot and root samples were extracted in methanol using an automated Buchi high pressure extractor while soil samples were extracted using a rotary shaking method. Extracts were profiled for unique secondary plant products acting as allelochemicals for weed suppression, specifically benzoxazinoids (BXs) (Fomsgaard et al. 2006), using liquid chromatograph coupled to a triple quadrupole mass spectrometer (UPLC-MS QQQ). In addition, non-targeted metabolomics analysis was performed to evaluate relative abundance of diverse metabolites using a quadrupole time-of-flight mass spectrometry (UPLC-MS QToF) platform. Metabolic profiling of wheat shoots, roots, and soils resulted in detection of up to 14 BXs including BX glycosides and other metabolites of interest (Adhikari et al. Tanwir et al. 2013). Both qualitative and quantitative differences in BXs were observed and were cultivar-, growth stage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BX concentration. The distribution of the secondary metabolites in wheat cultivar tissues suggest differential production of some key bioactive metabolites. Further metabolic profiling provided crucial information regarding crop metabolism, as well as the biosynthesis and release of metabolites associated with weed suppression in currently available commercial wheat cultivars, in contrast to weed suppressive rye (Secale cereale L.) and heritage wheat cultivars such as Federation, known for their potent ability to suppress weeds. This presentation will focus on the results of three years of field experimentation at two locations and predict which cultivars are best-suited for weed suppressive properties due to canopy architecture and allelopathic traits while maintaining high yield potential.",
keywords = "Weed suppression, metabolomics, residue, competition, resource allocation.",
author = "Mwendwa, {James M} and Paul Weston and Inge Fomsgaard and Laursen, {Bente B} and William Brown and Hanwen Wu and Jane Quinn and Jeffrey Weidenhamer and Razia Shaik and Leslie Weston",
year = "2017",
language = "English",
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Mwendwa, JM, Weston, P, Fomsgaard, I, Laursen, BB, Brown, W, Wu, H, Quinn, J, Weidenhamer, J, Shaik, R & Weston, L 2017, 'Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes' 8th World Congress of Allelopathy, Marseille, France, 24/07/17 - 28/07/17, .

Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes. / Mwendwa, James M; Weston, Paul; Fomsgaard, Inge; Laursen, Bente B; Brown, William; Wu, Hanwen; Quinn, Jane; Weidenhamer, Jeffrey; Shaik, Razia; Weston, Leslie.

2017. Abstract from 8th World Congress of Allelopathy, Marseille, France.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes

AU - Mwendwa, James M

AU - Weston, Paul

AU - Fomsgaard, Inge

AU - Laursen, Bente B

AU - Brown, William

AU - Wu, Hanwen

AU - Quinn, Jane

AU - Weidenhamer, Jeffrey

AU - Shaik, Razia

AU - Weston, Leslie

PY - 2017

Y1 - 2017

N2 - Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted in commercial fields in moderate to low rainfall zones at Wagga Wagga (572 mm average) and Condobolin (449 mm average) NSW, respectively in 2014, 2015 and 2016. At each experimental site, crop and/or weed growth were monitored at selected growth stages including tillering, vegetative, grain filling, harvest and after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected. All shoot and root samples were extracted in methanol using an automated Buchi high pressure extractor while soil samples were extracted using a rotary shaking method. Extracts were profiled for unique secondary plant products acting as allelochemicals for weed suppression, specifically benzoxazinoids (BXs) (Fomsgaard et al. 2006), using liquid chromatograph coupled to a triple quadrupole mass spectrometer (UPLC-MS QQQ). In addition, non-targeted metabolomics analysis was performed to evaluate relative abundance of diverse metabolites using a quadrupole time-of-flight mass spectrometry (UPLC-MS QToF) platform. Metabolic profiling of wheat shoots, roots, and soils resulted in detection of up to 14 BXs including BX glycosides and other metabolites of interest (Adhikari et al. Tanwir et al. 2013). Both qualitative and quantitative differences in BXs were observed and were cultivar-, growth stage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BX concentration. The distribution of the secondary metabolites in wheat cultivar tissues suggest differential production of some key bioactive metabolites. Further metabolic profiling provided crucial information regarding crop metabolism, as well as the biosynthesis and release of metabolites associated with weed suppression in currently available commercial wheat cultivars, in contrast to weed suppressive rye (Secale cereale L.) and heritage wheat cultivars such as Federation, known for their potent ability to suppress weeds. This presentation will focus on the results of three years of field experimentation at two locations and predict which cultivars are best-suited for weed suppressive properties due to canopy architecture and allelopathic traits while maintaining high yield potential.

AB - Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted in commercial fields in moderate to low rainfall zones at Wagga Wagga (572 mm average) and Condobolin (449 mm average) NSW, respectively in 2014, 2015 and 2016. At each experimental site, crop and/or weed growth were monitored at selected growth stages including tillering, vegetative, grain filling, harvest and after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected. All shoot and root samples were extracted in methanol using an automated Buchi high pressure extractor while soil samples were extracted using a rotary shaking method. Extracts were profiled for unique secondary plant products acting as allelochemicals for weed suppression, specifically benzoxazinoids (BXs) (Fomsgaard et al. 2006), using liquid chromatograph coupled to a triple quadrupole mass spectrometer (UPLC-MS QQQ). In addition, non-targeted metabolomics analysis was performed to evaluate relative abundance of diverse metabolites using a quadrupole time-of-flight mass spectrometry (UPLC-MS QToF) platform. Metabolic profiling of wheat shoots, roots, and soils resulted in detection of up to 14 BXs including BX glycosides and other metabolites of interest (Adhikari et al. Tanwir et al. 2013). Both qualitative and quantitative differences in BXs were observed and were cultivar-, growth stage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BX concentration. The distribution of the secondary metabolites in wheat cultivar tissues suggest differential production of some key bioactive metabolites. Further metabolic profiling provided crucial information regarding crop metabolism, as well as the biosynthesis and release of metabolites associated with weed suppression in currently available commercial wheat cultivars, in contrast to weed suppressive rye (Secale cereale L.) and heritage wheat cultivars such as Federation, known for their potent ability to suppress weeds. This presentation will focus on the results of three years of field experimentation at two locations and predict which cultivars are best-suited for weed suppressive properties due to canopy architecture and allelopathic traits while maintaining high yield potential.

KW - Weed suppression, metabolomics, residue, competition, resource allocation.

UR - https://wca2017.sciencesconf.org/

M3 - Abstract

ER -

Mwendwa JM, Weston P, Fomsgaard I, Laursen BB, Brown W, Wu H et al. Metabolic profiling for benzoxazinoids in weed-suppressive and early vigour wheat genotypes. 2017. Abstract from 8th World Congress of Allelopathy, Marseille, France.