Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes

James Mwendwa, Paul Weston, Inge Fomsgaard, Bente B Laursen, William Brown, Hanwen Wu, Jane Quinn, Jeffrey Weidenhamer, Leslie Weston

Research output: Other contribution to conferenceAbstract

Abstract

Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted in
moderate to low rainfall zones at Wagga Wagga (572
mm) and Condobolin (449 mm) NSW, respectively
in 2014–2016. At each experimental site, crop and/or
weed growth were monitored at selected growth stages
including tillering, vegetative, grain filling, harvest
and after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collected
for metabolomics profiling and biomass evaluation.
Plant tissue samples were extracted in methanol using
an automated Buchi high pressure extractor while soil
samples were extracted using a rotary shaker. Extracts
were filtered and specifically analysed for unique secondary metabolites or allelochemicals associated with
weed suppression, specifically benzoxazinoids (BXs),
using liquid chromatography coupled to quadrupole
time-of-flight mass spectrometry (UPLC-MS QToF).
Metabolic profiling of wheat shoots, roots, and soils
resulted in detection of up to 14 individual BXs including BX glycosides, lactones and hydroxamic acids of interest. Both qualitative and quantitative differences
in BXs were observed and were cultivar-, growth
stage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BX
concentration. Further metabolic profiling provided
crucial information regarding crop metabolism, as
well as the biosynthesis and release of metabolites
associated with weed suppression in currently available commercial wheat cultivars, in contrast to weed
suppressive rye (Secale cereale L.) and a heritage
wheat cultivar Federation, both recognised for their
potent ability to suppress weeds. We conclude that
certain commercial wheat cultivars maintained high
yield potential and were significantly more weed
suppressive, depending on year and location, due to
both their early growth habit and canopy architecture
as well as the release of BX metabolites into the
rhizosphere over time.
Original languageEnglish
Pages146
Number of pages1
Publication statusPublished - 2018
Event21st Australasian Weeds Conference 2018: Weed biosecurity - Protecting our future - Novotel Sydney Manly Pacific, Manly, Australia
Duration: 09 Sep 201812 Sep 2018
https://www.21awc.org.au/
http://caws.org.nz/old-site/awc_contents.php?yr=2018 (conference proceedings)
https://abercrombie.eventsair.com/QuickEventWebsitePortal/21awc/21awc-web/Agenda (conference program)

Conference

Conference21st Australasian Weeds Conference 2018
CountryAustralia
CityManly
Period09/09/1812/09/18
OtherThe Weed Society of New South Wales Inc., on behalf of the Council of Australasian Weed Societies Inc., will be hosting the 21st Australasian Weeds Conference in the popular Sydney beach side suburb of Manly from 9 - 12 September 2018.  An assortment of field trips will be hosted on Thursday 13 September 2018.

This biennial conference carries on a long tradition of bringing the weed management community together to discuss new developments and share information about cutting-edge and best weed management practices.

The conference attracts over 250 delegates from across Australasia and globally.  Delegates will come together to network with peers, engage with industry sponsors, listen and participate in presentations and field trips on a variety of topics including; 
- New technologies in weed management.
- Biological, mechanical, and chemical weed control and research.
- Herbicide resistance.
- Weeds of crops and pastures.
- Environmental weeds and Weeds of National Significance.
Internet address

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benzoxazinoids
metabolomics
vigor
weeds
wheat
genotype
cultivars
rhizosphere
crops
hydroxamic acids
rhizoplane
extractors
shoots
allelochemicals
Secale cereale
growth habit
tillering
lactones
filling period
rye

Cite this

Mwendwa, J., Weston, P., Fomsgaard, I., Laursen, B. B., Brown, W., Wu, H., ... Weston, L. (2018). Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes. 146. Abstract from 21st Australasian Weeds Conference 2018, Manly, Australia.
Mwendwa, James ; Weston, Paul ; Fomsgaard, Inge ; Laursen, Bente B ; Brown, William ; Wu, Hanwen ; Quinn, Jane ; Weidenhamer, Jeffrey ; Weston, Leslie. / Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes. Abstract from 21st Australasian Weeds Conference 2018, Manly, Australia.1 p.
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abstract = "Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted inmoderate to low rainfall zones at Wagga Wagga (572mm) and Condobolin (449 mm) NSW, respectivelyin 2014–2016. At each experimental site, crop and/orweed growth were monitored at selected growth stagesincluding tillering, vegetative, grain filling, harvestand after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collectedfor metabolomics profiling and biomass evaluation.Plant tissue samples were extracted in methanol usingan automated Buchi high pressure extractor while soilsamples were extracted using a rotary shaker. Extractswere filtered and specifically analysed for unique secondary metabolites or allelochemicals associated withweed suppression, specifically benzoxazinoids (BXs),using liquid chromatography coupled to quadrupoletime-of-flight mass spectrometry (UPLC-MS QToF).Metabolic profiling of wheat shoots, roots, and soilsresulted in detection of up to 14 individual BXs including BX glycosides, lactones and hydroxamic acids of interest. Both qualitative and quantitative differencesin BXs were observed and were cultivar-, growthstage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BXconcentration. Further metabolic profiling providedcrucial information regarding crop metabolism, aswell as the biosynthesis and release of metabolitesassociated with weed suppression in currently available commercial wheat cultivars, in contrast to weedsuppressive rye (Secale cereale L.) and a heritagewheat cultivar Federation, both recognised for theirpotent ability to suppress weeds. We conclude thatcertain commercial wheat cultivars maintained highyield potential and were significantly more weedsuppressive, depending on year and location, due toboth their early growth habit and canopy architectureas well as the release of BX metabolites into therhizosphere over time.",
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Mwendwa, J, Weston, P, Fomsgaard, I, Laursen, BB, Brown, W, Wu, H, Quinn, J, Weidenhamer, J & Weston, L 2018, 'Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes' 21st Australasian Weeds Conference 2018, Manly, Australia, 09/09/18 - 12/09/18, pp. 146.

Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes. / Mwendwa, James; Weston, Paul; Fomsgaard, Inge; Laursen, Bente B; Brown, William; Wu, Hanwen; Quinn, Jane; Weidenhamer, Jeffrey; Weston, Leslie.

2018. 146 Abstract from 21st Australasian Weeds Conference 2018, Manly, Australia.

Research output: Other contribution to conferenceAbstract

TY - CONF

T1 - Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes

AU - Mwendwa, James

AU - Weston, Paul

AU - Fomsgaard, Inge

AU - Laursen, Bente B

AU - Brown, William

AU - Wu, Hanwen

AU - Quinn, Jane

AU - Weidenhamer, Jeffrey

AU - Weston, Leslie

PY - 2018

Y1 - 2018

N2 - Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted inmoderate to low rainfall zones at Wagga Wagga (572mm) and Condobolin (449 mm) NSW, respectivelyin 2014–2016. At each experimental site, crop and/orweed growth were monitored at selected growth stagesincluding tillering, vegetative, grain filling, harvestand after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collectedfor metabolomics profiling and biomass evaluation.Plant tissue samples were extracted in methanol usingan automated Buchi high pressure extractor while soilsamples were extracted using a rotary shaker. Extractswere filtered and specifically analysed for unique secondary metabolites or allelochemicals associated withweed suppression, specifically benzoxazinoids (BXs),using liquid chromatography coupled to quadrupoletime-of-flight mass spectrometry (UPLC-MS QToF).Metabolic profiling of wheat shoots, roots, and soilsresulted in detection of up to 14 individual BXs including BX glycosides, lactones and hydroxamic acids of interest. Both qualitative and quantitative differencesin BXs were observed and were cultivar-, growthstage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BXconcentration. Further metabolic profiling providedcrucial information regarding crop metabolism, aswell as the biosynthesis and release of metabolitesassociated with weed suppression in currently available commercial wheat cultivars, in contrast to weedsuppressive rye (Secale cereale L.) and a heritagewheat cultivar Federation, both recognised for theirpotent ability to suppress weeds. We conclude thatcertain commercial wheat cultivars maintained highyield potential and were significantly more weedsuppressive, depending on year and location, due toboth their early growth habit and canopy architectureas well as the release of BX metabolites into therhizosphere over time.

AB - Replicated and randomised wheat (Triticum aestivum L.) cultivar trials were conducted inmoderate to low rainfall zones at Wagga Wagga (572mm) and Condobolin (449 mm) NSW, respectivelyin 2014–2016. At each experimental site, crop and/orweed growth were monitored at selected growth stagesincluding tillering, vegetative, grain filling, harvestand after crop harvest. In addition, shoots, roots, rhizoplane and bulk rhizosphere soil samples were collectedfor metabolomics profiling and biomass evaluation.Plant tissue samples were extracted in methanol usingan automated Buchi high pressure extractor while soilsamples were extracted using a rotary shaker. Extractswere filtered and specifically analysed for unique secondary metabolites or allelochemicals associated withweed suppression, specifically benzoxazinoids (BXs),using liquid chromatography coupled to quadrupoletime-of-flight mass spectrometry (UPLC-MS QToF).Metabolic profiling of wheat shoots, roots, and soilsresulted in detection of up to 14 individual BXs including BX glycosides, lactones and hydroxamic acids of interest. Both qualitative and quantitative differencesin BXs were observed and were cultivar-, growthstage- and location-dependent. Plant part and rhizosphere location (distance from root) also impacted BXconcentration. Further metabolic profiling providedcrucial information regarding crop metabolism, aswell as the biosynthesis and release of metabolitesassociated with weed suppression in currently available commercial wheat cultivars, in contrast to weedsuppressive rye (Secale cereale L.) and a heritagewheat cultivar Federation, both recognised for theirpotent ability to suppress weeds. We conclude thatcertain commercial wheat cultivars maintained highyield potential and were significantly more weedsuppressive, depending on year and location, due toboth their early growth habit and canopy architectureas well as the release of BX metabolites into therhizosphere over time.

KW - wheat

KW - benzoxazinoids

KW - Weed suppression

KW - rye

KW - crop metabolism

M3 - Abstract

SP - 146

ER -

Mwendwa J, Weston P, Fomsgaard I, Laursen BB, Brown W, Wu H et al. Metabolic profiling for benzoxazinoids in weed suppressive and early vigour wheat genotypes. 2018. Abstract from 21st Australasian Weeds Conference 2018, Manly, Australia.