Metabolomic approaches for the identification of flavonoids associated with weed suppression in selected Hardseeded annual pasture legumes

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Abstract

Background and aims: Weed suppressive potential of annual pasture legumes has been previously described, the mechanism of interference with weeds has not been clearly elucidated. We, therefore, aimed to delineate the role of secondary metabolites synthesized and released by pasture legumes through a series of studies to: 1) characterize key metabolites present in plant tissues, residue and the rhizosphere and 2) correlate their presence with weed suppressive properties. Methods: Field and in vitro experimentations coupled with targeted and non-targeted metabolic profiling was performed to evaluate the abundance of key phytotoxic metabolites using UHPLC QTOF-MS. Further, those metabolites strongly correlated with weed suppression and phytotoxicity were predicted by chemometric analyses and their concentration evaluated in field soils collected from the same legume site. Results: Field experimentation, methanolic extracts and dried residues of Biserrula pelecinus L. and Ornithopus compressus L. exhibited marked phytotoxicity in a series of laboratory experiments. Metabolic profiling revealed that both foliar tissues and rhizosphere soils of pasture legumes possessed a high abundance of flavonoids and their precursors. Chemometric analyses suggested the strong association of quercetin, isoquercetin, kaempferol, and kaempferol-7-O-glucoside with weed suppression under field conditions. Specifically, the abundance of quercetin and kaempferol was significantly higher in soils collected from established stands of biserrula and yellow serradella in contrast to arrowleaf, gland and subterranean clover. Conclusion: Current study provided an evidence for the role of certain annual legume-produced flavonoids in weed suppression in southern Australia and further insight into their localization and release in the soil rhizosphere.

Original languageEnglish
Pages (from-to)1-20
Number of pages20
JournalPlant and Soil
DOIs
Publication statusE-pub ahead of print - 30 Jul 2019

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forage legumes
metabolomics
weed
weed control
pasture
Ornithopus compressus
kaempferol
flavonoids
rhizosphere
chemometrics
weeds
phytotoxicity
metabolites
quercetin
soil
metabolite
legumes
Trifolium subterraneum
crossover interference
secondary metabolites

Cite this

@article{28ce27ecbb19468e8333037d4dd4495e,
title = "Metabolomic approaches for the identification of flavonoids associated with weed suppression in selected Hardseeded annual pasture legumes",
abstract = "Background and aims: Weed suppressive potential of annual pasture legumes has been previously described, the mechanism of interference with weeds has not been clearly elucidated. We, therefore, aimed to delineate the role of secondary metabolites synthesized and released by pasture legumes through a series of studies to: 1) characterize key metabolites present in plant tissues, residue and the rhizosphere and 2) correlate their presence with weed suppressive properties. Methods: Field and in vitro experimentations coupled with targeted and non-targeted metabolic profiling was performed to evaluate the abundance of key phytotoxic metabolites using UHPLC QTOF-MS. Further, those metabolites strongly correlated with weed suppression and phytotoxicity were predicted by chemometric analyses and their concentration evaluated in field soils collected from the same legume site. Results: Field experimentation, methanolic extracts and dried residues of Biserrula pelecinus L. and Ornithopus compressus L. exhibited marked phytotoxicity in a series of laboratory experiments. Metabolic profiling revealed that both foliar tissues and rhizosphere soils of pasture legumes possessed a high abundance of flavonoids and their precursors. Chemometric analyses suggested the strong association of quercetin, isoquercetin, kaempferol, and kaempferol-7-O-glucoside with weed suppression under field conditions. Specifically, the abundance of quercetin and kaempferol was significantly higher in soils collected from established stands of biserrula and yellow serradella in contrast to arrowleaf, gland and subterranean clover. Conclusion: Current study provided an evidence for the role of certain annual legume-produced flavonoids in weed suppression in southern Australia and further insight into their localization and release in the soil rhizosphere.",
keywords = "Annual hard-seeded pasture legumes, Chemometric analysis, Flavonoids, Metabolic profiling, Phytotoxicity, Root exudation, Weed suppression",
author = "Sajid Latif and Saliya Gurusinghe and Weston, {Paul A.} and Quinn, {Jane C.} and Piltz, {John W.} and Weston, {Leslie A.}",
year = "2019",
month = "7",
day = "30",
doi = "10.1007/s11104-019-04225-4",
language = "English",
pages = "1--20",
journal = "Plant and Soil: international journal on plant-soil relationships",
issn = "0032-079X",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Metabolomic approaches for the identification of flavonoids associated with weed suppression in selected Hardseeded annual pasture legumes

AU - Latif, Sajid

AU - Gurusinghe, Saliya

AU - Weston, Paul A.

AU - Quinn, Jane C.

AU - Piltz, John W.

AU - Weston, Leslie A.

PY - 2019/7/30

Y1 - 2019/7/30

N2 - Background and aims: Weed suppressive potential of annual pasture legumes has been previously described, the mechanism of interference with weeds has not been clearly elucidated. We, therefore, aimed to delineate the role of secondary metabolites synthesized and released by pasture legumes through a series of studies to: 1) characterize key metabolites present in plant tissues, residue and the rhizosphere and 2) correlate their presence with weed suppressive properties. Methods: Field and in vitro experimentations coupled with targeted and non-targeted metabolic profiling was performed to evaluate the abundance of key phytotoxic metabolites using UHPLC QTOF-MS. Further, those metabolites strongly correlated with weed suppression and phytotoxicity were predicted by chemometric analyses and their concentration evaluated in field soils collected from the same legume site. Results: Field experimentation, methanolic extracts and dried residues of Biserrula pelecinus L. and Ornithopus compressus L. exhibited marked phytotoxicity in a series of laboratory experiments. Metabolic profiling revealed that both foliar tissues and rhizosphere soils of pasture legumes possessed a high abundance of flavonoids and their precursors. Chemometric analyses suggested the strong association of quercetin, isoquercetin, kaempferol, and kaempferol-7-O-glucoside with weed suppression under field conditions. Specifically, the abundance of quercetin and kaempferol was significantly higher in soils collected from established stands of biserrula and yellow serradella in contrast to arrowleaf, gland and subterranean clover. Conclusion: Current study provided an evidence for the role of certain annual legume-produced flavonoids in weed suppression in southern Australia and further insight into their localization and release in the soil rhizosphere.

AB - Background and aims: Weed suppressive potential of annual pasture legumes has been previously described, the mechanism of interference with weeds has not been clearly elucidated. We, therefore, aimed to delineate the role of secondary metabolites synthesized and released by pasture legumes through a series of studies to: 1) characterize key metabolites present in plant tissues, residue and the rhizosphere and 2) correlate their presence with weed suppressive properties. Methods: Field and in vitro experimentations coupled with targeted and non-targeted metabolic profiling was performed to evaluate the abundance of key phytotoxic metabolites using UHPLC QTOF-MS. Further, those metabolites strongly correlated with weed suppression and phytotoxicity were predicted by chemometric analyses and their concentration evaluated in field soils collected from the same legume site. Results: Field experimentation, methanolic extracts and dried residues of Biserrula pelecinus L. and Ornithopus compressus L. exhibited marked phytotoxicity in a series of laboratory experiments. Metabolic profiling revealed that both foliar tissues and rhizosphere soils of pasture legumes possessed a high abundance of flavonoids and their precursors. Chemometric analyses suggested the strong association of quercetin, isoquercetin, kaempferol, and kaempferol-7-O-glucoside with weed suppression under field conditions. Specifically, the abundance of quercetin and kaempferol was significantly higher in soils collected from established stands of biserrula and yellow serradella in contrast to arrowleaf, gland and subterranean clover. Conclusion: Current study provided an evidence for the role of certain annual legume-produced flavonoids in weed suppression in southern Australia and further insight into their localization and release in the soil rhizosphere.

KW - Annual hard-seeded pasture legumes

KW - Chemometric analysis

KW - Flavonoids

KW - Metabolic profiling

KW - Phytotoxicity

KW - Root exudation

KW - Weed suppression

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U2 - 10.1007/s11104-019-04225-4

DO - 10.1007/s11104-019-04225-4

M3 - Article

SP - 1

EP - 20

JO - Plant and Soil: international journal on plant-soil relationships

JF - Plant and Soil: international journal on plant-soil relationships

SN - 0032-079X

ER -