TY - JOUR
T1 - Metataxonomics, metagenomics and metabolomics analysis of the influence of temperature modification in full-scale anaerobic digesters
AU - Puig-Castellví, Francesc
AU - Midoux, Cédric
AU - Guenne, Angéline
AU - Conteau, Delphine
AU - Franchi, Oscar
AU - Bureau, Chrystelle
AU - Madigou, Céline
AU - Jouan-Rimbaud Bouveresse, Delphine
AU - Kroff, Pablo
AU - Mazéas, Laurent
AU - Rutledge, Douglas N.
AU - Gaval, Gilberte
AU - Chapleur, Olivier
N1 - Funding Information:
This work is part of the DIGESTOMIC project funded by the National Research 470 Agency (ANR-16-CE05-0014)
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/2
Y1 - 2022/2
N2 - Full-scale anaerobic digesters’ performance is regulated by modifying their operational conditions, but little is known about how these modifications affect their microbiome. In this work, we monitored two originally mesophilic (35 °C) full-scale anaerobic digesters during 476 days. One digester was submitted to sub-mesophilic (25 °C) conditions between days 123 and 373. We characterized the effect of temperature modification using a multi-omics (metataxonomics, metagenomics, and metabolomics) approach. The metataxonomics and metagenomics results revealed that the lower temperature allowed a substantial increase of the sub-dominant bacterial population, destabilizing the microbial community equilibrium and reducing the biogas production. After restoring the initial mesophilic temperature, the bacterial community manifested resilience in terms of microbial structure and functional activity. The metabolomic signature of the sub-mesophilic acclimation was characterized by a rise of amino acids and short peptides, suggesting a protein degradation activity not directed towards biogas production.
AB - Full-scale anaerobic digesters’ performance is regulated by modifying their operational conditions, but little is known about how these modifications affect their microbiome. In this work, we monitored two originally mesophilic (35 °C) full-scale anaerobic digesters during 476 days. One digester was submitted to sub-mesophilic (25 °C) conditions between days 123 and 373. We characterized the effect of temperature modification using a multi-omics (metataxonomics, metagenomics, and metabolomics) approach. The metataxonomics and metagenomics results revealed that the lower temperature allowed a substantial increase of the sub-dominant bacterial population, destabilizing the microbial community equilibrium and reducing the biogas production. After restoring the initial mesophilic temperature, the bacterial community manifested resilience in terms of microbial structure and functional activity. The metabolomic signature of the sub-mesophilic acclimation was characterized by a rise of amino acids and short peptides, suggesting a protein degradation activity not directed towards biogas production.
KW - Chemometrics
KW - Full-scale
KW - Metabolomics
KW - Metagenomics
KW - Sub-mesophilic
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U2 - 10.1016/j.biortech.2021.126612
DO - 10.1016/j.biortech.2021.126612
M3 - Article
C2 - 34954354
AN - SCOPUS:85122186324
VL - 346
JO - Biological Wastes
JF - Biological Wastes
SN - 0960-8524
M1 - 126612
ER -