TY - JOUR
T1 - Methanol, ethyl acetate and n-hexane extracts of Tragia involucrata L. leaves exhibit anxiolytic, sedative and analgesic activity in Swiss albino mice
AU - Islam, Md Shofiqul
AU - Sana, Samiron
AU - Haque, Md Ehsanul
AU - Rahman, S. M.Mushiur
AU - Samad, Abdus
AU - Al Noman, Abdullah
AU - Alam, Rahat
AU - Rana, Sohel
AU - Meem, Rima Islam
AU - Mondol, Debashish
AU - Islam, Md Shariful
AU - Islam, Md Torikul
AU - Mazumder, Kishor
N1 - Funding Information:
The authors are grateful to Dr. Niranjan Kumar Sana, Professor, Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi-6205, Bangladesh for providing his thoughts and guidance. The authors are also grateful to Pharmacology laboratory, Department of Pharmacy, Jashore University of Science and Technology, Jashore- 7408, Bangladesh and Asia Arsenic Network, Jessore ?Benapole Road, Jessore, Bangladesh, for providing their laboratory facilities to conduct this research.
Publisher Copyright:
© 2020 The Author(s)
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - IntroductionTragia involucrata
L. have been utilized as traditional medicine in Indian subcontinent
for the treatment of numerous illnesses such as inflammation, pain and
skin infection. In this current study we sought to assess the
anxiolytic, sedative and analgesic activity of Tragia involucrata L. leaves extract.Materials and methodsWe
first performed a phytochemical screening test of the leaves extracts
following standard phytochemical screening protocols. We next examined
the anxiolytic and sedative activity of crude methanol (TIME), ethyl
acetate (TIEAE) and n-Hexane (TIHE) extract of Tragia involucrata
L. leaves using mouse behavioral models such as elevated plus-maze test
and pentobarbital-induced sleeping time test, respectively. Likewise,
we evaluated the analgesic activity using acetic acid induced writhing
test and formalin induced paw licking test. Additionally, we performed a
quantitative analysis of heavy metals content of Tragia involucrata L. leaves by overnight digestion in concentrated nitric acid (HNO3).ResultsPhytochemical
screening demonstrated that TIME, TIEAE and TIHE contain flavonoids,
alkaloids, tannins, phenols, terpenoids and sterols. Administration of
these extracts resulted in higher number of open arm entry, lower number
of close arm entry and higher time spent in open arm compared to
control treatment (p < 0.05). Moreover, these treatments decreased
the onset of sleep time and increased the duration of sleep compared to
control treated mice (all p < 0.05). Likewise, extracts treated mice
exhibited decreased number of writhing as well as lower acute phase and
late phase duration compared to control treatment (all p < 0.05). The
average level of As and Fe in Tragia involucrata L. leaves was 5.16 ± 0.012 ppm and 2.76 ± 0.015 ppm, respectively.ConclusionResults from this study support that Tragia involucrata L. leaves extracts exhibit an anxiolytic, sedative and analgesic activity in mice.
AB - IntroductionTragia involucrata
L. have been utilized as traditional medicine in Indian subcontinent
for the treatment of numerous illnesses such as inflammation, pain and
skin infection. In this current study we sought to assess the
anxiolytic, sedative and analgesic activity of Tragia involucrata L. leaves extract.Materials and methodsWe
first performed a phytochemical screening test of the leaves extracts
following standard phytochemical screening protocols. We next examined
the anxiolytic and sedative activity of crude methanol (TIME), ethyl
acetate (TIEAE) and n-Hexane (TIHE) extract of Tragia involucrata
L. leaves using mouse behavioral models such as elevated plus-maze test
and pentobarbital-induced sleeping time test, respectively. Likewise,
we evaluated the analgesic activity using acetic acid induced writhing
test and formalin induced paw licking test. Additionally, we performed a
quantitative analysis of heavy metals content of Tragia involucrata L. leaves by overnight digestion in concentrated nitric acid (HNO3).ResultsPhytochemical
screening demonstrated that TIME, TIEAE and TIHE contain flavonoids,
alkaloids, tannins, phenols, terpenoids and sterols. Administration of
these extracts resulted in higher number of open arm entry, lower number
of close arm entry and higher time spent in open arm compared to
control treatment (p < 0.05). Moreover, these treatments decreased
the onset of sleep time and increased the duration of sleep compared to
control treated mice (all p < 0.05). Likewise, extracts treated mice
exhibited decreased number of writhing as well as lower acute phase and
late phase duration compared to control treatment (all p < 0.05). The
average level of As and Fe in Tragia involucrata L. leaves was 5.16 ± 0.012 ppm and 2.76 ± 0.015 ppm, respectively.ConclusionResults from this study support that Tragia involucrata L. leaves extracts exhibit an anxiolytic, sedative and analgesic activity in mice.
KW - Analgesic and heavy metal study
KW - Anxiolytic
KW - Phytochemical screening
KW - Sedative
KW - Tragia involucrata L.
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U2 - 10.1016/j.heliyon.2020.e05814
DO - 10.1016/j.heliyon.2020.e05814
M3 - Article
C2 - 33426350
AN - SCOPUS:85098711617
VL - 7
SP - 1
EP - 9
JO - Heliyon
JF - Heliyon
SN - 2405-8440
IS - 1
M1 - e05814
ER -