Background and Aims: The beginning of a grapevine inflorescence commences in spring with floral evocation, defined as the irreversible changes in the shoot apical meristem committing the plant to flower formation. To identify evocation genes, repeated sampling of shoots was necessary; however, utilising fixed vineyard plants was unsuitable. To obtain samples, methods were required for: (i) continual production of smaller plants that could be transferred to a laboratory; and (ii) repeated induction of budburst year-round in these plants. The aim of this presentation is to detail why these methods were required and describe how the methods were applied to produce plants in an environmentally controlled greenhouse. Methods and Results: Green cuttings were taken from field sylleptic shoots, in spring and summer, and from greenhouse growing grapevine shoots. A method to grow cuttings in an environmentally controlled greenhouse was developed. The strike rate for field mid-spring and greenhouse vines (83 and 80%) showed no significant difference, whereas the strike rate of field late summer cuttings was 13%; a significant difference. Also developed was a method for repeated induction of budburst on greenhouse vines, initially grown from green cuttings. Conclusions: A method is described to successfully grow grapevine green cuttings in an environmentally controlled greenhouse. Also described is a method to induce repeated budburst, at least six times per year, in mature greenhouse-grown vines. Significance of the Study: For the first time, a protocol to produce grapevine plants in environmentally controlled greenhouses, from green cuttings, has been reported, advancing research on grapevine development in season 1.