Methylation of CpG Sites in Exon 2 of the Bcl-2 Gene Occurs in Colorectal Carcinoma

Wendy Babidge, Lisa Butler, Mark Burton, Prudence Cowled

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Abstract

BACKGROUND: Aberrant bcl-2 expression frequently occurs in colorectal carcinoma. The current study investigated if CpG sites in bcl-2 were methylated in colorectal carcinoma and if methylation correlated with loss of expression of bcl-2 mRNA. METHODS: Methylation was assessed in 23 matched normal mucosae and colonic carcinomas by Southern blotting with methylation-sensitive enzymes. Expression of bcl-2 mRNA was assessed by Northern blotting. RESULTS: A SacII site in exon 2 of the bcl-2 gene was methylated in 5 carcinomas, plus an adjacent HpaII sites in 1 tumour. SacII site in the bcl-2 promoter were not methylated. Elevated levels of bcl-2 mRNA were detected in 3 carcinomas, 5 showed decreased expression and 4 were unchanged. CONCLUSIONS: De novo methylation of CpG sites in exon 2 of the bcl-2 gene occurs during the development of colorectal carcinoma. However, there was no relationship between expression of bc1-2 mRNA and methylation of specific CpG sites.
Original languageEnglish
Pages (from-to)2809-2814
Number of pages6
JournalAnticancer Research
Volume21
Issue number4A
Publication statusPublished - 2001

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    Babidge, W., Butler, L., Burton, M., & Cowled, P. (2001). Methylation of CpG Sites in Exon 2 of the Bcl-2 Gene Occurs in Colorectal Carcinoma. Anticancer Research, 21(4A), 2809-2814.