Microfluidic technology for the generation of cell spheroids and their applications

Raja K. Vadivelu; Harshad Kamble; Muhammad J.A. Shiddiky; Nam Trung Nguyen

doi:10.3390/mi8040094

Microfluidic technology for the generation of cell spheroids and their applications

Raja K. Vadivelu, Harshad Kamble, Muhammad J.A. Shiddiky, Nam Trung Nguyen

Griffith University

Research output: Contribution to journal › Review article › peer-review

99 Citations (Scopus)

26 Downloads (Pure)

Abstract

A three-dimensional (3D) tissue model has significant advantages over the conventional two-dimensional (2D) model. A 3D model mimics the relevant in-vivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, tissue engineering, and the investigation of disease pathology. The present reviews highlight the recent advances and the development of microfluidics based methods for the generation of cell spheroids. The paper emphasizes on the application of microfluidic technology for tissue engineering including the formation of multicellular spheroids (MCS). Further, the paper discusses the recent technical advances in the integration of microfluidic devices for MCS-based high-throughput drug screening. The review compares the various microfluidic techniques and finally provides a perspective for the future opportunities in this research area.

Original language	English
Article number	94
Pages (from-to)	1-23
Number of pages	23
Journal	Micromachines
Volume	8
Issue number	4
Early online date	Mar 2017
DOIs	https://doi.org/10.3390/mi8040094
Publication status	Published - Apr 2017

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10.3390/mi8040094Licence: CC BY

349042491_published articleFinal published version, 720 KBLicence: CC BY

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title = "Microfluidic technology for the generation of cell spheroids and their applications",

abstract = "A three-dimensional (3D) tissue model has significant advantages over the conventional two-dimensional (2D) model. A 3D model mimics the relevant in-vivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, tissue engineering, and the investigation of disease pathology. The present reviews highlight the recent advances and the development of microfluidics based methods for the generation of cell spheroids. The paper emphasizes on the application of microfluidic technology for tissue engineering including the formation of multicellular spheroids (MCS). Further, the paper discusses the recent technical advances in the integration of microfluidic devices for MCS-based high-throughput drug screening. The review compares the various microfluidic techniques and finally provides a perspective for the future opportunities in this research area.",

keywords = "BioMEMS, Cell spheroids, Microfluidics, Three-dimensional cell culture, Tissue engineering",

author = "Vadivelu, {Raja K.} and Harshad Kamble and Shiddiky, {Muhammad J.A.} and Nguyen, {Nam Trung}",

note = "Funding Information: We acknowledge the Australian Research Council for the support through the grant DP170100277. Publisher Copyright: {\textcopyright} 2017 by the authors.",

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AU - Shiddiky, Muhammad J.A.

AU - Nguyen, Nam Trung

PY - 2017/4

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N2 - A three-dimensional (3D) tissue model has significant advantages over the conventional two-dimensional (2D) model. A 3D model mimics the relevant in-vivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, tissue engineering, and the investigation of disease pathology. The present reviews highlight the recent advances and the development of microfluidics based methods for the generation of cell spheroids. The paper emphasizes on the application of microfluidic technology for tissue engineering including the formation of multicellular spheroids (MCS). Further, the paper discusses the recent technical advances in the integration of microfluidic devices for MCS-based high-throughput drug screening. The review compares the various microfluidic techniques and finally provides a perspective for the future opportunities in this research area.

AB - A three-dimensional (3D) tissue model has significant advantages over the conventional two-dimensional (2D) model. A 3D model mimics the relevant in-vivo physiological conditions, allowing a cell culture to serve as an effective tool for drug discovery, tissue engineering, and the investigation of disease pathology. The present reviews highlight the recent advances and the development of microfluidics based methods for the generation of cell spheroids. The paper emphasizes on the application of microfluidic technology for tissue engineering including the formation of multicellular spheroids (MCS). Further, the paper discusses the recent technical advances in the integration of microfluidic devices for MCS-based high-throughput drug screening. The review compares the various microfluidic techniques and finally provides a perspective for the future opportunities in this research area.

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Microfluidic technology for the generation of cell spheroids and their applications

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