Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

Meng Xiao; Xin Fan; Xin Xin Chen; He Wang; Li Zhang; Zhi Peng Xu; Timothy Kudinha; Fanrong Kong; Ying Chun Xu

doi:10.1128/JCM.02830-15

Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

Meng Xiao, Xin Fan, Xin Xin Chen, He Wang, Li Zhang, Zhi Peng Xu, Timothy Kudinha, Fanrong Kong, Ying Chun Xu

Nursing, Paramedicine and Healthcare Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

8 Downloads (Pure)

Abstract

Forty-two putative Cryptococcus laurentii isolates identified by the Vitek 2 system were collected in China. The gold standard, internal transcribed spacer (ITS) sequencing, confirmed that only two isolates were genuine C. laurentii. Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry was able to identify the C. laurentii isolates with an expanded custom database.

Original language	English
Pages (from-to)	226-229
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	54
Issue number	1
DOIs	https://doi.org/10.1128/JCM.02830-15
Publication status	Published - Jan 2016

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Xiao, M., Fan, X., Chen, X. X., Wang, H., Zhang, L., Xu, Z. P., Kudinha, T., Kong, F., & Xu, Y. C. (2016). Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories. Journal of Clinical Microbiology, 54(1), 226-229. https://doi.org/10.1128/JCM.02830-15

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title = "Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories",

abstract = "Forty-two putative Cryptococcus laurentii isolates identified by the Vitek 2 system were collected in China. The gold standard, internal transcribed spacer (ITS) sequencing, confirmed that only two isolates were genuine C. laurentii. Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometry was able to identify the C. laurentii isolates with an expanded custom database.",

author = "Meng Xiao and Xin Fan and Chen, {Xin Xin} and He Wang and Li Zhang and Xu, {Zhi Peng} and Timothy Kudinha and Fanrong Kong and Xu, {Ying Chun}",

note = "Includes bibliographical references.",

year = "2016",

month = jan,

doi = "10.1128/JCM.02830-15",

language = "English",

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Xiao, M, Fan, X, Chen, XX, Wang, H, Zhang, L, Xu, ZP, Kudinha, T, Kong, F & Xu, YC 2016, 'Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories', Journal of Clinical Microbiology, vol. 54, no. 1, pp. 226-229. https://doi.org/10.1128/JCM.02830-15

Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories. / Xiao, Meng; Fan, Xin; Chen, Xin Xin et al.
In: Journal of Clinical Microbiology, Vol. 54, No. 1, 01.2016, p. 226-229.

Research output: Contribution to journal › Article › peer-review

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T2 - Challenges for clinical mycology laboratories

AU - Xiao, Meng

AU - Fan, Xin

AU - Chen, Xin Xin

AU - Wang, He

AU - Zhang, Li

AU - Xu, Zhi Peng

AU - Kudinha, Timothy

AU - Kong, Fanrong

AU - Xu, Ying Chun

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Xiao M, Fan X, Chen XX, Wang H, Zhang L, Xu ZP et al. Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories. Journal of Clinical Microbiology. 2016 Jan;54(1):226-229. doi: 10.1128/JCM.02830-15

Misidentification of a rare species, cryptococcus laurentii, by commonly used commercial biochemical methods and matrix-assisted laser desorption ionization-time of flight mass spectrometry systems: Challenges for clinical mycology laboratories

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