Modification of polypyrrole nanowires array with platinum nanoparticles and glucose oxidase for fabrication of a novel glucose biosensor

Guangqing Xu, Samuel B. Adeloju, Yucheng Wu, Xinyi Zhang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A novel glucose biosensor, based on the modification of well-aligned polypyrrole nanowires array (PPyNWA) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. The distinct differences in the electrochemical properties of PPyNWA-GOx, PPyNWA-PtNPs, and PPyNWA-PtNPs-GOx electrodes were revealed by cyclic voltammetry. In particular, the results obtained for PPyNWA-PtNPs-GOx biosensor showed evidence of direct electron transfer due mainly to modification with PtNPs. Optimum fabrication of the PPyNWA-PtNPs-GOx biosensor for both potentiometric and amperometric detection of glucose were achieved with 0.2M pyrrole, applied current density of 0.1mAcm-2, polymerization time of 600s, cyclic deposition of PtNPs from -200mV to 200mV, scan rate of 50mVs-1, and 20 cycles. A sensitivity of 40.5mV/decade and a linear range of 10μM to 1000μM (R2=0.9936) were achieved for potentiometric detection, while for amperometric detection a sensitivity of 34.7μAcm-2mM-1 at an applied potential of 700mV and a linear range of 0.1-9mM (R2=0.9977) were achieved. In terms of achievable detection limit, potentiometric detection achieved 5.6μM of glucose, while amperometric detection achieved 27.7μM.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalAnalytica Chimica Acta
Volume755
DOIs
Publication statusPublished - 28 Nov 2012

Fingerprint

Nanowires
Glucose Oxidase
Biosensing Techniques
Platinum
platinum
Biosensors
Nanoparticles
glucose
Glucose
Fabrication
Pyrroles
polypyrrole
nanoparticle
Electrochemical properties
Polymerization
Adsorption
Cyclic voltammetry
Limit of Detection
density current
polymerization

Cite this

@article{47fd14e6c5c94ec68d967639ced76de4,
title = "Modification of polypyrrole nanowires array with platinum nanoparticles and glucose oxidase for fabrication of a novel glucose biosensor",
abstract = "A novel glucose biosensor, based on the modification of well-aligned polypyrrole nanowires array (PPyNWA) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. The distinct differences in the electrochemical properties of PPyNWA-GOx, PPyNWA-PtNPs, and PPyNWA-PtNPs-GOx electrodes were revealed by cyclic voltammetry. In particular, the results obtained for PPyNWA-PtNPs-GOx biosensor showed evidence of direct electron transfer due mainly to modification with PtNPs. Optimum fabrication of the PPyNWA-PtNPs-GOx biosensor for both potentiometric and amperometric detection of glucose were achieved with 0.2M pyrrole, applied current density of 0.1mAcm-2, polymerization time of 600s, cyclic deposition of PtNPs from -200mV to 200mV, scan rate of 50mVs-1, and 20 cycles. A sensitivity of 40.5mV/decade and a linear range of 10μM to 1000μM (R2=0.9936) were achieved for potentiometric detection, while for amperometric detection a sensitivity of 34.7μAcm-2mM-1 at an applied potential of 700mV and a linear range of 0.1-9mM (R2=0.9977) were achieved. In terms of achievable detection limit, potentiometric detection achieved 5.6μM of glucose, while amperometric detection achieved 27.7μM.",
keywords = "Biosensor, Electrochemical detection, Glucose, PPy nanowires, Pt nanoparticles",
author = "Guangqing Xu and Adeloju, {Samuel B.} and Yucheng Wu and Xinyi Zhang",
year = "2012",
month = "11",
day = "28",
doi = "10.1016/j.aca.2012.09.037",
language = "English",
volume = "755",
pages = "100--107",
journal = "Analytica Chimica Acta",
issn = "0003-2670",
publisher = "Elsevier",

}

TY - JOUR

T1 - Modification of polypyrrole nanowires array with platinum nanoparticles and glucose oxidase for fabrication of a novel glucose biosensor

AU - Xu, Guangqing

AU - Adeloju, Samuel B.

AU - Wu, Yucheng

AU - Zhang, Xinyi

PY - 2012/11/28

Y1 - 2012/11/28

N2 - A novel glucose biosensor, based on the modification of well-aligned polypyrrole nanowires array (PPyNWA) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. The distinct differences in the electrochemical properties of PPyNWA-GOx, PPyNWA-PtNPs, and PPyNWA-PtNPs-GOx electrodes were revealed by cyclic voltammetry. In particular, the results obtained for PPyNWA-PtNPs-GOx biosensor showed evidence of direct electron transfer due mainly to modification with PtNPs. Optimum fabrication of the PPyNWA-PtNPs-GOx biosensor for both potentiometric and amperometric detection of glucose were achieved with 0.2M pyrrole, applied current density of 0.1mAcm-2, polymerization time of 600s, cyclic deposition of PtNPs from -200mV to 200mV, scan rate of 50mVs-1, and 20 cycles. A sensitivity of 40.5mV/decade and a linear range of 10μM to 1000μM (R2=0.9936) were achieved for potentiometric detection, while for amperometric detection a sensitivity of 34.7μAcm-2mM-1 at an applied potential of 700mV and a linear range of 0.1-9mM (R2=0.9977) were achieved. In terms of achievable detection limit, potentiometric detection achieved 5.6μM of glucose, while amperometric detection achieved 27.7μM.

AB - A novel glucose biosensor, based on the modification of well-aligned polypyrrole nanowires array (PPyNWA) with Pt nanoparticles (PtNPs) and subsequent surface adsorption of glucose oxidase (GOx), is described. The distinct differences in the electrochemical properties of PPyNWA-GOx, PPyNWA-PtNPs, and PPyNWA-PtNPs-GOx electrodes were revealed by cyclic voltammetry. In particular, the results obtained for PPyNWA-PtNPs-GOx biosensor showed evidence of direct electron transfer due mainly to modification with PtNPs. Optimum fabrication of the PPyNWA-PtNPs-GOx biosensor for both potentiometric and amperometric detection of glucose were achieved with 0.2M pyrrole, applied current density of 0.1mAcm-2, polymerization time of 600s, cyclic deposition of PtNPs from -200mV to 200mV, scan rate of 50mVs-1, and 20 cycles. A sensitivity of 40.5mV/decade and a linear range of 10μM to 1000μM (R2=0.9936) were achieved for potentiometric detection, while for amperometric detection a sensitivity of 34.7μAcm-2mM-1 at an applied potential of 700mV and a linear range of 0.1-9mM (R2=0.9977) were achieved. In terms of achievable detection limit, potentiometric detection achieved 5.6μM of glucose, while amperometric detection achieved 27.7μM.

KW - Biosensor

KW - Electrochemical detection

KW - Glucose

KW - PPy nanowires

KW - Pt nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=84869085765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869085765&partnerID=8YFLogxK

U2 - 10.1016/j.aca.2012.09.037

DO - 10.1016/j.aca.2012.09.037

M3 - Article

VL - 755

SP - 100

EP - 107

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

SN - 0003-2670

ER -