Molecular and microscopic characterization of a novel Eastern grey kangaroopox virus genome directly from a clinical sample

Subir Sarker, Hayley K. Roberts, Naomie Tidd, Shayne Ault, Georgia Ladmore, Andrew Peters, Jade K. Forwood, Karla Helbig, Shane R. Raidal

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Abstract

Poxviruses are large DNA viruses with varying zoonotic potential, and are recognised in a broad range of wildlife. Although poxviruses have been detected in kangaroos, their genetic relationships to poxviruses in other animals and humans is not well understood. Here, we present a novel genome sequence of a marsupial poxvirus, the Eastern grey kangaroopox virus (EKPV-NSW), isolated from a wild eastern grey kangaroo. In the present study, histopathologically confirmed epidermal pox lesions were used to recover the full-length viral genome and perform electron microscopic analysis, with both immature virions and intracellular mature virions detected. Subsequent analysis of the EKPV-NSW genome demonstrated the highest degree of sequence similarity with EKPV-SC strain (91.51%), followed by WKPV-WA (87.93%), and MOCV1 (44.05%). The novel EKPV-NSW complete genome encompasses most of the chordopoxviruses protein coding genes (138) that are required for genome replication and expression, with only three essential protein coding genes being absent. The novel EKPV-NSW is missing 28 predicted genes compared to the recently isolated EKPV-SC, and carries 21 additional unique genes, encoding unknown proteins. Phylogenetic and recombination analyses showed EKPV-NSW to be the distinct available candidate genome of chordopoxviruses.

Original languageEnglish
Article number16472
Pages (from-to)1-13
Number of pages13
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 28 Nov 2017

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Poxviridae
Genome
Viruses
Macropodidae
Virion
Marsupialia
Proteins
DNA Viruses
Viral Genome
Zoonoses
Genetic Recombination
Genes
Electrons

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Sarker, Subir ; Roberts, Hayley K. ; Tidd, Naomie ; Ault, Shayne ; Ladmore, Georgia ; Peters, Andrew ; Forwood, Jade K. ; Helbig, Karla ; Raidal, Shane R. / Molecular and microscopic characterization of a novel Eastern grey kangaroopox virus genome directly from a clinical sample. In: Scientific Reports. 2017 ; Vol. 7, No. 1. pp. 1-13.
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Molecular and microscopic characterization of a novel Eastern grey kangaroopox virus genome directly from a clinical sample. / Sarker, Subir; Roberts, Hayley K.; Tidd, Naomie; Ault, Shayne; Ladmore, Georgia; Peters, Andrew; Forwood, Jade K.; Helbig, Karla; Raidal, Shane R.

In: Scientific Reports, Vol. 7, No. 1, 16472, 28.11.2017, p. 1-13.

Research output: Contribution to journalArticle

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