TY - JOUR
T1 - Molecular characterisation of the monogenea parasites of blue mackerel Scomber australasicus (Perciformes: Scombridae) in Australian waters
AU - Hossen, Md Shafaet
AU - Barton, Diane P.
AU - Wassens, Skye
AU - Shamsi, Shokoofeh
N1 - Publisher Copyright:
© 2022 The Authors
PY - 2022/12
Y1 - 2022/12
N2 - This study describes the occurrence and molecular identification of Monogenea from blue mackerel Scomber australasicus (Cuvier) (Perciformes: Scombridae), an edible fish, from Australian waters. Previous studies have provided either morphological or genetic results, whereas this study combines both methods of species identification. A total of 50 fish sourced from the waters off the south-eastern Australian coastline were examined and 71 Monogenea were recovered from the gills. The overall prevalence, mean intensity, and mean abundance were 64%, 2.22, and 1.42, respectively. Monogenea were initially classified morphologically as five species belonging to two families. Family Mazocraeidae was represented by Kuhnia scombri (Kuhn, 1829) Sproston, 1945, K. scombercolias Nasir & Fuentes Zambrano, 1983 and Pseudokuhnia minor (Goto, 1894) Rohde & Watson, 1985 and family Gastrocotylidae by Gastrocotyle kurra Unnithan, 1968 and Allogastrocotyle bivaginalis Nasir & Fuentes Zambrano, 1983. Molecular identification of Monogenea was conducted through sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The host S. australasicus was barcoded (cox1) to confirm the specific identity. There was no comparable sequence available in GenBank for K. scombercolias. Also, limited sequences were available in GenBank for the gastrocotylid Monogenea identified in this study. However, phylogenetic analyses of cox1 sequences of the Monogenea identified in this study clustered according to their familial groups. Gastrocotyle kurra and A. bivaginalis were identified for the first-time on S. australasicus in Australian waters. This study provides the first sequencing of cox1 gene for K. scombercolias. The outcomes of the study provide a basis for future Monogenea research in Australian waters, as well as for other Scomber spp.
AB - This study describes the occurrence and molecular identification of Monogenea from blue mackerel Scomber australasicus (Cuvier) (Perciformes: Scombridae), an edible fish, from Australian waters. Previous studies have provided either morphological or genetic results, whereas this study combines both methods of species identification. A total of 50 fish sourced from the waters off the south-eastern Australian coastline were examined and 71 Monogenea were recovered from the gills. The overall prevalence, mean intensity, and mean abundance were 64%, 2.22, and 1.42, respectively. Monogenea were initially classified morphologically as five species belonging to two families. Family Mazocraeidae was represented by Kuhnia scombri (Kuhn, 1829) Sproston, 1945, K. scombercolias Nasir & Fuentes Zambrano, 1983 and Pseudokuhnia minor (Goto, 1894) Rohde & Watson, 1985 and family Gastrocotylidae by Gastrocotyle kurra Unnithan, 1968 and Allogastrocotyle bivaginalis Nasir & Fuentes Zambrano, 1983. Molecular identification of Monogenea was conducted through sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The host S. australasicus was barcoded (cox1) to confirm the specific identity. There was no comparable sequence available in GenBank for K. scombercolias. Also, limited sequences were available in GenBank for the gastrocotylid Monogenea identified in this study. However, phylogenetic analyses of cox1 sequences of the Monogenea identified in this study clustered according to their familial groups. Gastrocotyle kurra and A. bivaginalis were identified for the first-time on S. australasicus in Australian waters. This study provides the first sequencing of cox1 gene for K. scombercolias. The outcomes of the study provide a basis for future Monogenea research in Australian waters, as well as for other Scomber spp.
KW - Fish
KW - Monogenea
KW - cox1
KW - Mazocraeidae
KW - Gastrocotylidae
KW - Australia
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U2 - 10.1016/j.ijppaw.2022.08.007
DO - 10.1016/j.ijppaw.2022.08.007
M3 - Article
C2 - 36119441
SN - 2213-2244
VL - 19
SP - 115
EP - 127
JO - International Journal For Parasitology: Parasites And Wildlife
JF - International Journal For Parasitology: Parasites And Wildlife
ER -