Molecularly imprinted polymer membranes and thin films for the separation and sensing of biomacromolecules

Reinhard I. Boysen, Lachlan J. Schwarz, Dan V. Nicolau, Milton T. W. Hearn

Research output: Contribution to journalReview articlepeer-review

73 Citations (Scopus)


This review describes recent advances associated with the development of surface imprinting methods for the synthesis of polymeric membranes and thin films, which possess the capability to selectively and specifically recognize biomacromolecules, such as proteins and single- and double-stranded DNA, employing “epitope” or “whole molecule” approaches. Synthetic procedures to create different molecularly imprinted polymer membranes or thin films are discussed, including grafting/in situ polymerization, drop-, dip-, or spin-coating procedures, electropolymerization as well as micro-contact or stamp lithography imprinting methods. Highly sensitive techniques for surface characterization and analyte detection are described, encompassing luminescence and fluorescence spectroscopy, X-ray photoelectron spectroscopy, FTIR spectroscopy, surface-enhanced Raman spectroscopy, atomic force microscopy, quartz crystal microbalance analysis, cyclic voltammetry, and surface plasmon resonance. These developments are providing new avenues to produce bioelectronic sensors and new ways to explore through advanced separation science procedures complex phenomena associated with the origins of biorecognition in nature.
Original languageEnglish
Pages (from-to)314-335
Number of pages22
JournalJournal of Separation Science
Issue number1
Early online dateOct 2016
Publication statusPublished - 01 Jan 2017


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