TY - JOUR
T1 - Monomer conversion, release and cytotoxicity analyses of experimental GDMA-P based adhesives
AU - Sousa-Lima, Rodolfo
AU - Justo-Fernandes, Ana
AU - Kaizer, Marina
AU - Isolan, Cristina
AU - Moraes, Rafael
AU - Aragão, Cícero
AU - Barboza, Carlos
AU - Assunção, Isauremi
AU - Borges, Boniek
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2021/3
Y1 - 2021/3
N2 - This study aimed to evaluate the effects of a photoinitiator system and acidic monomer (GDMA-P) concentrations on self-etch experimental dental adhesive degree of conversion (DC), monomer release and cytotoxicity. Three monomer blends containing different GDMA-P/HEMA/UDMA ratios were produced: 1/3/3; 2/3/2; and 3/3/1. CQ + EDMAB; BAPO or CQ + BAPO + EDMAB were used as photoinitiator systems. DFI was added as co-initiator, and a water/ethanol mixture was used as solvent. DC was evaluated by Fourier transform infrared spectroscopy (FTIR) (n = 10). Monomer release was identified by ultra-high performance liquid chromatography (UHPLC) (n = 6). The Alamar Blue assay was used to test NIH-3T3 fibroblast cell viability (n = 8). A two-way ANOVA and Tukey post-hoc test were used for the data analyses (p < 0.05). Experimental adhesives presenting higher GDMA-P concentrations displayed an overall statistically lower DC, less monomer release and increased cell viability (p < 0.05). BAPO resulted in increased DC and lower monomer release rates than CQ only (p < 0.05). Low GDMA-P concentrations associated to BAPO generated an adhesive system with improved conversion and both lower monomer release rates and cytotoxicity.
AB - This study aimed to evaluate the effects of a photoinitiator system and acidic monomer (GDMA-P) concentrations on self-etch experimental dental adhesive degree of conversion (DC), monomer release and cytotoxicity. Three monomer blends containing different GDMA-P/HEMA/UDMA ratios were produced: 1/3/3; 2/3/2; and 3/3/1. CQ + EDMAB; BAPO or CQ + BAPO + EDMAB were used as photoinitiator systems. DFI was added as co-initiator, and a water/ethanol mixture was used as solvent. DC was evaluated by Fourier transform infrared spectroscopy (FTIR) (n = 10). Monomer release was identified by ultra-high performance liquid chromatography (UHPLC) (n = 6). The Alamar Blue assay was used to test NIH-3T3 fibroblast cell viability (n = 8). A two-way ANOVA and Tukey post-hoc test were used for the data analyses (p < 0.05). Experimental adhesives presenting higher GDMA-P concentrations displayed an overall statistically lower DC, less monomer release and increased cell viability (p < 0.05). BAPO resulted in increased DC and lower monomer release rates than CQ only (p < 0.05). Low GDMA-P concentrations associated to BAPO generated an adhesive system with improved conversion and both lower monomer release rates and cytotoxicity.
KW - Infrared spectroscopy
KW - Photopolymerization
KW - Self-etch adhesive
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U2 - 10.1016/j.ijadhadh.2020.102792
DO - 10.1016/j.ijadhadh.2020.102792
M3 - Article
AN - SCOPUS:85097881972
SN - 0143-7496
VL - 105
JO - International Journal of Adhesion and Adhesives
JF - International Journal of Adhesion and Adhesives
M1 - 102792
ER -