Myeloid progenitor surface antigen identified by monoclonal antibody

K F Bradstock; E J Favaloro; A Kabral; A Kerr; W G Hughes; E Musgrove

doi:10.1111/j.1365-2141.1985.tb04055.x

Myeloid progenitor surface antigen identified by monoclonal antibody

K F Bradstock, E J Favaloro, A Kabral, A Kerr, W G Hughes, E Musgrove

Dentistry and Medical Sciences

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Abstract

A mouse monoclonal antibody, WM-15, has been developed which reacts with a human myeloid lineage-restricted cell surface antigen. WM-15, an IgG1 antibody, reacts with a mean of 3.8% of normal bone marrow mononuclear cells, identifying predominantly promyelocytes and myeloblasts, and in fluorescence-activated cell sorting experiments produces enrichment of bone marrow granulocyte-macrophage progenitor cells. Normal mature monocytes and granulocytes are weakly labelled by WM-15, but other haemopoietic cells including erythroblasts and all lymphoid cells are unreactive. The myeloid specificity of this antibody is highlighted by its reactivity with myeloid leukaemia cell lines and 65% of cases of acute myeloid leukaemia, while lymphoid cell lines and leukaemias are WM-15 negative. WM-15 appears to be a useful reagent for further investigation of normal and abnormal myelopoiesis.

Original language	English
Pages (from-to)	11-20
Number of pages	10
Journal	British Journal of Haematology
Volume	61
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-2141.1985.tb04055.x
Publication status	Published - Sept 1985

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title = "Myeloid progenitor surface antigen identified by monoclonal antibody",

abstract = "A mouse monoclonal antibody, WM-15, has been developed which reacts with a human myeloid lineage-restricted cell surface antigen. WM-15, an IgG1 antibody, reacts with a mean of 3.8% of normal bone marrow mononuclear cells, identifying predominantly promyelocytes and myeloblasts, and in fluorescence-activated cell sorting experiments produces enrichment of bone marrow granulocyte-macrophage progenitor cells. Normal mature monocytes and granulocytes are weakly labelled by WM-15, but other haemopoietic cells including erythroblasts and all lymphoid cells are unreactive. The myeloid specificity of this antibody is highlighted by its reactivity with myeloid leukaemia cell lines and 65% of cases of acute myeloid leukaemia, while lymphoid cell lines and leukaemias are WM-15 negative. WM-15 appears to be a useful reagent for further investigation of normal and abnormal myelopoiesis.",

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author = "Bradstock, {K F} and Favaloro, {E J} and A Kabral and A Kerr and Hughes, {W G} and E Musgrove",

year = "1985",

month = sep,

doi = "10.1111/j.1365-2141.1985.tb04055.x",

language = "English",

volume = "61",

pages = "11--20",

journal = "British Journal of Haematology",

issn = "0007-1048",

publisher = "Wiley-Blackwell",

number = "1",

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TY - JOUR

T1 - Myeloid progenitor surface antigen identified by monoclonal antibody

AU - Bradstock, K F

AU - Favaloro, E J

AU - Kabral, A

AU - Kerr, A

AU - Hughes, W G

AU - Musgrove, E

PY - 1985/9

Y1 - 1985/9

N2 - A mouse monoclonal antibody, WM-15, has been developed which reacts with a human myeloid lineage-restricted cell surface antigen. WM-15, an IgG1 antibody, reacts with a mean of 3.8% of normal bone marrow mononuclear cells, identifying predominantly promyelocytes and myeloblasts, and in fluorescence-activated cell sorting experiments produces enrichment of bone marrow granulocyte-macrophage progenitor cells. Normal mature monocytes and granulocytes are weakly labelled by WM-15, but other haemopoietic cells including erythroblasts and all lymphoid cells are unreactive. The myeloid specificity of this antibody is highlighted by its reactivity with myeloid leukaemia cell lines and 65% of cases of acute myeloid leukaemia, while lymphoid cell lines and leukaemias are WM-15 negative. WM-15 appears to be a useful reagent for further investigation of normal and abnormal myelopoiesis.

AB - A mouse monoclonal antibody, WM-15, has been developed which reacts with a human myeloid lineage-restricted cell surface antigen. WM-15, an IgG1 antibody, reacts with a mean of 3.8% of normal bone marrow mononuclear cells, identifying predominantly promyelocytes and myeloblasts, and in fluorescence-activated cell sorting experiments produces enrichment of bone marrow granulocyte-macrophage progenitor cells. Normal mature monocytes and granulocytes are weakly labelled by WM-15, but other haemopoietic cells including erythroblasts and all lymphoid cells are unreactive. The myeloid specificity of this antibody is highlighted by its reactivity with myeloid leukaemia cell lines and 65% of cases of acute myeloid leukaemia, while lymphoid cell lines and leukaemias are WM-15 negative. WM-15 appears to be a useful reagent for further investigation of normal and abnormal myelopoiesis.

KW - Animals

KW - Antibodies, Monoclonal/immunology

KW - Antibody Specificity

KW - Antigens, Neoplasm/analysis

KW - Antigens, Surface/analysis

KW - Blood Cells/immunology

KW - Bone Marrow/immunology

KW - Cell Line

KW - Hematopoietic Stem Cells/immunology

KW - Humans

KW - Leukemia/immunology

KW - Mice

KW - Mice, Inbred BALB C

U2 - 10.1111/j.1365-2141.1985.tb04055.x

DO - 10.1111/j.1365-2141.1985.tb04055.x

M3 - Article

C2 - 4052321

SN - 0007-1048

VL - 61

SP - 11

EP - 20

JO - British Journal of Haematology

JF - British Journal of Haematology

IS - 1

ER -

Myeloid progenitor surface antigen identified by monoclonal antibody

Abstract

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