Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation

Rui Yang, Yuqian Liu, Yunjing Gao, Zhiying Yang, Siqi Zhao, Yongjin Wang, Chris Blanchard, Zhongkai Zhou

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105M−1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalFood Research International
Volume108
Early online date01 Mar 2018
DOIs
Publication statusPublished - 01 Jun 2018

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