Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation

Rui Yang, Yuqian Liu, Yunjing Gao, Zhiying Yang, Siqi Zhao, Yongjin Wang, Chris Blanchard, Zhongkai Zhou

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105M−1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalFood Research International
Volume108
Early online date01 Mar 2018
DOIs
Publication statusPublished - 01 Jun 2018

Fingerprint

epigallocatechin
Apoferritins
Chitosan
ferritin
Ferritins
encapsulation
chitosan
Digestion
digestion
Pepsin A
Static Electricity
Soybeans
Trypsin
Gastrointestinal Tract
Polyacrylamide Gel Electrophoresis
Seeds
epigallocatechin gallate
electrostatic interactions
Food
pepsin

Cite this

Yang, Rui ; Liu, Yuqian ; Gao, Yunjing ; Yang, Zhiying ; Zhao, Siqi ; Wang, Yongjin ; Blanchard, Chris ; Zhou, Zhongkai. / Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation. In: Food Research International. 2018 ; Vol. 108. pp. 1-7.
@article{9f495e5ed13f47ef9a3539a9cd542a72,
title = "Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation",
abstract = "In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105M−1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69{\%} (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.",
keywords = "Absorption by Caco-2 cell, Digestion, Epigallocatechin gallate, Ferritin, Nanocarrier",
author = "Rui Yang and Yuqian Liu and Yunjing Gao and Zhiying Yang and Siqi Zhao and Yongjin Wang and Chris Blanchard and Zhongkai Zhou",
year = "2018",
month = "6",
day = "1",
doi = "10.1016/j.foodres.2018.02.074",
language = "English",
volume = "108",
pages = "1--7",
journal = "Food Research International",
issn = "0963-9969",
publisher = "Elsevier",

}

Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation. / Yang, Rui; Liu, Yuqian; Gao, Yunjing; Yang, Zhiying; Zhao, Siqi; Wang, Yongjin; Blanchard, Chris; Zhou, Zhongkai.

In: Food Research International, Vol. 108, 01.06.2018, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nano-encapsulation of epigallocatechin gallate in the ferritin-chitosan double shells: Simulated digestion and absorption evaluation

AU - Yang, Rui

AU - Liu, Yuqian

AU - Gao, Yunjing

AU - Yang, Zhiying

AU - Zhao, Siqi

AU - Wang, Yongjin

AU - Blanchard, Chris

AU - Zhou, Zhongkai

PY - 2018/6/1

Y1 - 2018/6/1

N2 - In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105M−1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.

AB - In this work, a double shell material chitosan (CS)-recombinant soybean seed H-2 ferritin (H-2F) was fabricated to encapsulate epigallocatechin gallate (EGCG) molecules. EGCG-loaded H-2F complex (EHF) was firstly prepared by taking advantages of the reversible self-assembly of the ferritin, and the EHF-CS composite (EHFC) was fabricated by electrostatic interactions with binding number n of (4.1 ± 0.11) and binding constant K of ((5.3 ± 0.2) × 105M−1), respectively. It was calculated that about 12.6 of EGCG molecules can be encapsulated in one H-2F ferritin cage with an encapsulation efficiency of 9.69% (w/w). SDS-PAGE analysis indicated that the CS binding to H-2F could inhibit ferritin degradation by pepsin and trypsin; the stability of EGCG molecules in EHFC was also significantly improved in simulated gastrointestinal tract. In addition, the chitosan-ferritin double shells were beneficial for the transport of EGCG across the Caco-2 monolayer model based on ferritin uptake. This work demonstrates a novel method to promote stabilization and absorption of food bioactive molecules.

KW - Absorption by Caco-2 cell

KW - Digestion

KW - Epigallocatechin gallate

KW - Ferritin

KW - Nanocarrier

U2 - 10.1016/j.foodres.2018.02.074

DO - 10.1016/j.foodres.2018.02.074

M3 - Article

VL - 108

SP - 1

EP - 7

JO - Food Research International

JF - Food Research International

SN - 0963-9969

ER -