Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas

David G. Burns; Peter H. Janssen; Takashi Itoh; Hiroaki Minegishi; Ron Usami; Masahiro Kamekura; Michael Dyall-Smith

doi:10.1099/ijs.0.010132-0

Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas

David G. Burns, Peter H. Janssen, Takashi Itoh, Hiroaki Minegishi, Ron Usami, Masahiro Kamekura, Michael Dyall-Smith

Science and Health

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18-20 % (w/v) NaCl, pH 6.0-8.5 and 37-40 degrees C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA-DNA hybridization values between Nmn. pharaonis DSM 2160(T) and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11(T) (=JCM 14361(T) =CECT 7526(T) =DSM 18674(T)). An emended description of the genus Natronomonas is also provided.

Original language	English
Pages (from-to)	1173-1176
Number of pages	4
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	60
Issue number	5
DOIs	https://doi.org/10.1099/ijs.0.010132-0
Publication status	Published - May 2010

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Burns, D. G., Janssen, P. H., Itoh, T., Minegishi, H., Usami, R., Kamekura, M., & Dyall-Smith, M. (2010). Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas. International Journal of Systematic and Evolutionary Microbiology, 60(5), 1173-1176. https://doi.org/10.1099/ijs.0.010132-0

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title = "Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas",

abstract = "Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18-20 % (w/v) NaCl, pH 6.0-8.5 and 37-40 degrees C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA-DNA hybridization values between Nmn. pharaonis DSM 2160(T) and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11(T) (=JCM 14361(T) =CECT 7526(T) =DSM 18674(T)). An emended description of the genus Natronomonas is also provided.",

keywords = "Archaea, Extremophile, Hypersaline, Taxonomy",

author = "Burns, {David G.} and Janssen, {Peter H.} and Takashi Itoh and Hiroaki Minegishi and Ron Usami and Masahiro Kamekura and Michael Dyall-Smith",

note = "Imported on 12 Apr 2017 - DigiTool details were: month (773h) = May 2010; Journal title (773t) = International Journal of Systematic and Evolutionary Microbiology. ISSNs: 1466-5026; ",

year = "2010",

month = may,

doi = "10.1099/ijs.0.010132-0",

language = "English",

volume = "60",

pages = "1173--1176",

journal = "International Journal of Systematic Bacteriology",

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publisher = "Society for General Microbiology",

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Burns, DG, Janssen, PH, Itoh, T, Minegishi, H, Usami, R, Kamekura, M & Dyall-Smith, M 2010, 'Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas', International Journal of Systematic and Evolutionary Microbiology, vol. 60, no. 5, pp. 1173-1176. https://doi.org/10.1099/ijs.0.010132-0

Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas. / Burns, David G.; Janssen, Peter H.; Itoh, Takashi et al.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 60, No. 5, 05.2010, p. 1173-1176.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas

AU - Burns, David G.

AU - Janssen, Peter H.

AU - Itoh, Takashi

AU - Minegishi, Hiroaki

AU - Usami, Ron

AU - Kamekura, Masahiro

AU - Dyall-Smith, Michael

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = May 2010; Journal title (773t) = International Journal of Systematic and Evolutionary Microbiology. ISSNs: 1466-5026;

PY - 2010/5

Y1 - 2010/5

N2 - Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18-20 % (w/v) NaCl, pH 6.0-8.5 and 37-40 degrees C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA-DNA hybridization values between Nmn. pharaonis DSM 2160(T) and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11(T) (=JCM 14361(T) =CECT 7526(T) =DSM 18674(T)). An emended description of the genus Natronomonas is also provided.

AB - Two isolates of non-alkaliphilic, extremely halophilic archaea, with very similar characteristics, were recovered from a marine solar saltern crystallizer. Cells were pleomorphic, motile and Gram-stain-negative and grew on a limited range of carbon sources, with pyruvate being the best substrate. Optimum growth occurred at 18-20 % (w/v) NaCl, pH 6.0-8.5 and 37-40 degrees C. Both isolates possessed typical archaeal lipids, and their 16S rRNA gene sequences were 99.8 % identical. Phylogenetic tree reconstructions indicated that they were most closely related to the haloalkaliphile Natronomonas pharaonis (97.5 % similarity to the type strain), but the different phenotypic properties and low DNA-DNA hybridization values between Nmn. pharaonis DSM 2160(T) and the two isolates suggested that they represent a novel species within the genus Natronomonas. The name Natronomonas moolapensis sp. nov. is proposed for these isolates, with the type strain being 8.8.11(T) (=JCM 14361(T) =CECT 7526(T) =DSM 18674(T)). An emended description of the genus Natronomonas is also provided.

KW - Archaea

KW - Extremophile

KW - Hypersaline

KW - Taxonomy

U2 - 10.1099/ijs.0.010132-0

DO - 10.1099/ijs.0.010132-0

M3 - Article

SN - 1070-6259

VL - 60

SP - 1173

EP - 1176

JO - International Journal of Systematic Bacteriology

JF - International Journal of Systematic Bacteriology

IS - 5

ER -

Natronomonas moolapensis sp. nov., non-alkaliphilic isolates recovered from a solar saltern crystallizer pond, and emended description of the genus Natronomonas

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