Numerous protocols for the isolation of bovine aortic endothelial cells have been described in the previous literature. However, these protocols prevent researchers from obtaining the pure population of endothelial cells. Thus, this study aimed to develop a new and economical method for the isolation of pure endothelial cells by introducing a new strategy to the enzymatic digestion method proposed by previous researchers.
Ataollahi, F., Pingguan-Murphy, B., Moradi, A., Wan Abbas, W. A. B., Chua, K. H., & Abu Osman, N. A. (2014). New method for the isolation of endothelial cells from large vessels. Cytotherapy, 16(8), 1145-1152. https://doi.org/10.1016/j.jcyt.2014.01.010