Next generation testing of herbicide resistance: Rapid detection, multiple herbicides, a single assay

Aaron Preston, James Pratley

    Research output: Book chapter/Published conference paperConference paperpeer-review


    Annual ryegrass is the major weed in Australian farming systems costing farmers $96 million annually, with control primarily by herbicides. This weed has evolved resistance to many herbicides making control extremely difficult with herbicide-based management programs. Using the genotyping technology DArTseq, a genetic assay has been developed that detects resistance to multiple herbicides in a single test within weeks of field observation. Over 350 individual ryegrass plants were screened with one or more herbicides (diclofop-methyl, chlorsulfuron and glyphosate) using conventional glasshouse based resistance testing procedures. DNA from these samples was then analysed with DArTseq, and herbicide resistance predicted based on the presence/absence of genetic markers. DArTseq analysis predicted herbicide resistance to each of these herbicides with 50–59% accuracy relative to glasshouse testing. This assay surpasses other resistance testing methods which require individual tests, a 9-month timeframe for results, and offers a new tool to combat resistance.
    Original languageEnglish
    Title of host publicationProceedings of the 21st Australian weeds conference
    EditorsStephen Johnson, Leslie Weston, Hanwen Wu, Bruce Auld
    Place of PublicationBoolaroo, Australia
    PublisherWeed Society of NSW
    Number of pages5
    ISBN (Electronic)9780995415911
    ISBN (Print)9781510888470
    Publication statusPublished - 2018
    Event21st Australasian Weeds Conference (2018) - Novotel Sydney Manly Pacific, Sydney, Australia
    Duration: 09 Sep 201813 Sep 2018 (conference proceedings) (conference program)


    Conference21st Australasian Weeds Conference (2018)
    Abbreviated titleWeed biosecurity - Protecting our future
    OtherThe Weed Society of New South Wales Inc., on behalf of the Council of Australasian Weed Societies Inc., will be hosting the 21st Australasian Weeds Conference in the popular Sydney beach side suburb of Manly from 9 - 12 September 2018.  An assortment of field trips will be hosted on Thursday 13 September 2018.
    This biennial conference carries on a long tradition of bringing the weed management community together to discuss new developments and share information about cutting-edge and best weed management practices.
    The conference attracts over 250 delegates from across Australasia and globally.  Delegates will come together to network with peers, engage with industry sponsors, listen and participate in presentations and field trips on a variety of topics including; 
    - New technologies in weed management.
    - Biological, mechanical, and chemical weed control and research.
    - Herbicide resistance.
    - Weeds of crops and pastures.
    - Environmental weeds and Weeds of National Significance.
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