TY - JOUR
T1 - Novel microsatellite markers reveal multiple origins of Botryosphaeria dothidea causing the Chinese grapevine trunk disease
AU - Manawasinghe, Ishara S.
AU - Zhang, Wei
AU - Li, Xinghong
AU - Zhao, Wensheng
AU - Chethana, K. W.Thilini
AU - Xu, Jianping
AU - Chen, Zhen
AU - Dissanayaka, Asha J.
AU - Mugnai, Laura
AU - Úrbez-Torres, José R.
AU - Savocchia, Sandra
AU - Hyde, Kevin D.
AU - Yan, Jiye
N1 - Includes bibliographical references.
PY - 2018/6
Y1 - 2018/6
N2 - Botryosphaeria dothidea causes diseases in many different hosts worldwide. This species has become the most prominent fungal taxon causing grapevine trunk disease in China, with a recent disease outbreak. To reveal the genetic diversity and explore the origin of B. dothidea, six novel microsatellite markers were developed and used for the genotyping of 151 isolates obtained from China and overseas. The results demonstrated the high genetic diversity of the B. dothidea populations. Bayesian cluster analysis separated the total B. dothidea isolates into five genetic populations. B. dothidea isolates from Chinese grapevines were observed to share alleles with isolates from different hosts within China and from grapevines growing overseas, indicating both endemic host shifts and exotic introduction. In addition, unique pathogen genotypes were identified in Chinese grapevine isolates. Hence, we infer that B. dothidea isolates from multiple origins are contributing to the dieback and canker outbreak currently occurring in China.
AB - Botryosphaeria dothidea causes diseases in many different hosts worldwide. This species has become the most prominent fungal taxon causing grapevine trunk disease in China, with a recent disease outbreak. To reveal the genetic diversity and explore the origin of B. dothidea, six novel microsatellite markers were developed and used for the genotyping of 151 isolates obtained from China and overseas. The results demonstrated the high genetic diversity of the B. dothidea populations. Bayesian cluster analysis separated the total B. dothidea isolates into five genetic populations. B. dothidea isolates from Chinese grapevines were observed to share alleles with isolates from different hosts within China and from grapevines growing overseas, indicating both endemic host shifts and exotic introduction. In addition, unique pathogen genotypes were identified in Chinese grapevine isolates. Hence, we infer that B. dothidea isolates from multiple origins are contributing to the dieback and canker outbreak currently occurring in China.
KW - Botryosphaeriaceae
KW - Fungal pathogens
KW - Genetic diversity
KW - Microsatellite genotyping
KW - Population structure
KW - SSR
KW - Vitis
UR - https://www.scopus.com/pages/publications/85045751955
UR - https://www.scopus.com/inward/citedby.url?scp=85045751955&partnerID=8YFLogxK
U2 - 10.1016/j.funeco.2018.02.004
DO - 10.1016/j.funeco.2018.02.004
M3 - Article
AN - SCOPUS:85045751955
SN - 1754-5048
VL - 33
SP - 134
EP - 142
JO - Fungal Ecology
JF - Fungal Ecology
ER -
