Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

Kaiser Dawood, JAT Morgan, F Busfield, M Srivastava, TI Fletcher, J. Sambono, LA Jackson, B Venus, AW Phlibey, AE Kew-Tabor

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The roles and epidemiological features of tick-borne protozoans are not well elicited in wildlife. Babesia spp. are documented in many domestic animals, including cattle, horses, pigs, dogs and cats. Three cases affecting eastern grey kangaroos are described. The kangaroos exhibited neurological signs, depression and marked anaemia, and microscopic examination of blood smears revealed intraerythrocytic piroplasms. One to seven intraerythrocytic spherical, oval, pyriform and irregularly-shaped parasites consistent with Babesia spp. were seen in the blood smears and the percentage of infected erythrocytes was estimated to be approximately 7% in each case. Data suggest that the tick vector for this kangaroo Babesia sp. is a Haemaphysalis species. For Case 2, ultrastructural examination of the erythrocytes of the renal capillaries showed parasites resembling Babesia spp. and 18 of 33 erythrocytes were infected. DNA sequencing of the amplified 18S rDNA confirmed that the observed intraerythrocytic piroplasms belong to the genus Babesia. The phylogenetic position of this new kangaroo Babesia sp. (de novo Babesia macropus), as a sister species to the new Australian woylie Babesia sp., suggests a close affinity to the described Afroâ€Â'Eurasian species Babesia orientalis and Babesia occultans suggesting perhaps a common ancestor for the Babesia in kangaroos.
Original languageEnglish
Pages (from-to)54-61
Number of pages8
JournalInternational Journal For Parasitology: Parasites And Wildlife
Volume2
Issue number1
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Macropus giganteus
Macropodidae
Babesia
Observation
Piroplasmida
erythrocytes
Erythrocytes
Ticks
ticks
Parasites
Macropus
Haemaphysalis
parasites
blood
Domestic Animals
Ribosomal DNA
DNA Sequence Analysis
domestic animals
anemia
Horses

Cite this

Dawood, Kaiser ; Morgan, JAT ; Busfield, F ; Srivastava, M ; Fletcher, TI ; Sambono, J. ; Jackson, LA ; Venus, B ; Phlibey, AW ; Kew-Tabor, AE. / Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia. In: International Journal For Parasitology: Parasites And Wildlife. 2013 ; Vol. 2, No. 1. pp. 54-61.
@article{a4107c559dc14658bc5caebb53b3406c,
title = "Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia",
abstract = "The roles and epidemiological features of tick-borne protozoans are not well elicited in wildlife. Babesia spp. are documented in many domestic animals, including cattle, horses, pigs, dogs and cats. Three cases affecting eastern grey kangaroos are described. The kangaroos exhibited neurological signs, depression and marked anaemia, and microscopic examination of blood smears revealed intraerythrocytic piroplasms. One to seven intraerythrocytic spherical, oval, pyriform and irregularly-shaped parasites consistent with Babesia spp. were seen in the blood smears and the percentage of infected erythrocytes was estimated to be approximately 7{\%} in each case. Data suggest that the tick vector for this kangaroo Babesia sp. is a Haemaphysalis species. For Case 2, ultrastructural examination of the erythrocytes of the renal capillaries showed parasites resembling Babesia spp. and 18 of 33 erythrocytes were infected. DNA sequencing of the amplified 18S rDNA confirmed that the observed intraerythrocytic piroplasms belong to the genus Babesia. The phylogenetic position of this new kangaroo Babesia sp. (de novo Babesia macropus), as a sister species to the new Australian woylie Babesia sp., suggests a close affinity to the described Afro{\~A}¢{\^A}€{\^A}'Eurasian species Babesia orientalis and Babesia occultans suggesting perhaps a common ancestor for the Babesia in kangaroos.",
author = "Kaiser Dawood and JAT Morgan and F Busfield and M Srivastava and TI Fletcher and J. Sambono and LA Jackson and B Venus and AW Phlibey and AE Kew-Tabor",
note = "Imported on 12 Apr 2017 - DigiTool details were: month (773h) = December, 2013; Journal title (773t) = International Journal for Parasitology: Parasites and Wildlife. ISSNs: 2213-2244;",
year = "2013",
month = "12",
doi = "10.1016/j.ijppaw.2012.12.001",
language = "English",
volume = "2",
pages = "54--61",
journal = "International Journal For Parasitology: Parasites And Wildlife",
issn = "2213-2244",
publisher = "Elsevier BV",
number = "1",

}

Dawood, K, Morgan, JAT, Busfield, F, Srivastava, M, Fletcher, TI, Sambono, J, Jackson, LA, Venus, B, Phlibey, AW & Kew-Tabor, AE 2013, 'Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia', International Journal For Parasitology: Parasites And Wildlife, vol. 2, no. 1, pp. 54-61. https://doi.org/10.1016/j.ijppaw.2012.12.001

Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia. / Dawood, Kaiser; Morgan, JAT; Busfield, F; Srivastava, M; Fletcher, TI; Sambono, J.; Jackson, LA; Venus, B; Phlibey, AW; Kew-Tabor, AE.

In: International Journal For Parasitology: Parasites And Wildlife, Vol. 2, No. 1, 12.2013, p. 54-61.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Observation of a novel Babesia spp. in Eastern Grey Kangaroos (Macropus giganteus) in Australia

AU - Dawood, Kaiser

AU - Morgan, JAT

AU - Busfield, F

AU - Srivastava, M

AU - Fletcher, TI

AU - Sambono, J.

AU - Jackson, LA

AU - Venus, B

AU - Phlibey, AW

AU - Kew-Tabor, AE

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = December, 2013; Journal title (773t) = International Journal for Parasitology: Parasites and Wildlife. ISSNs: 2213-2244;

PY - 2013/12

Y1 - 2013/12

N2 - The roles and epidemiological features of tick-borne protozoans are not well elicited in wildlife. Babesia spp. are documented in many domestic animals, including cattle, horses, pigs, dogs and cats. Three cases affecting eastern grey kangaroos are described. The kangaroos exhibited neurological signs, depression and marked anaemia, and microscopic examination of blood smears revealed intraerythrocytic piroplasms. One to seven intraerythrocytic spherical, oval, pyriform and irregularly-shaped parasites consistent with Babesia spp. were seen in the blood smears and the percentage of infected erythrocytes was estimated to be approximately 7% in each case. Data suggest that the tick vector for this kangaroo Babesia sp. is a Haemaphysalis species. For Case 2, ultrastructural examination of the erythrocytes of the renal capillaries showed parasites resembling Babesia spp. and 18 of 33 erythrocytes were infected. DNA sequencing of the amplified 18S rDNA confirmed that the observed intraerythrocytic piroplasms belong to the genus Babesia. The phylogenetic position of this new kangaroo Babesia sp. (de novo Babesia macropus), as a sister species to the new Australian woylie Babesia sp., suggests a close affinity to the described Afroâ€Â'Eurasian species Babesia orientalis and Babesia occultans suggesting perhaps a common ancestor for the Babesia in kangaroos.

AB - The roles and epidemiological features of tick-borne protozoans are not well elicited in wildlife. Babesia spp. are documented in many domestic animals, including cattle, horses, pigs, dogs and cats. Three cases affecting eastern grey kangaroos are described. The kangaroos exhibited neurological signs, depression and marked anaemia, and microscopic examination of blood smears revealed intraerythrocytic piroplasms. One to seven intraerythrocytic spherical, oval, pyriform and irregularly-shaped parasites consistent with Babesia spp. were seen in the blood smears and the percentage of infected erythrocytes was estimated to be approximately 7% in each case. Data suggest that the tick vector for this kangaroo Babesia sp. is a Haemaphysalis species. For Case 2, ultrastructural examination of the erythrocytes of the renal capillaries showed parasites resembling Babesia spp. and 18 of 33 erythrocytes were infected. DNA sequencing of the amplified 18S rDNA confirmed that the observed intraerythrocytic piroplasms belong to the genus Babesia. The phylogenetic position of this new kangaroo Babesia sp. (de novo Babesia macropus), as a sister species to the new Australian woylie Babesia sp., suggests a close affinity to the described Afroâ€Â'Eurasian species Babesia orientalis and Babesia occultans suggesting perhaps a common ancestor for the Babesia in kangaroos.

U2 - 10.1016/j.ijppaw.2012.12.001

DO - 10.1016/j.ijppaw.2012.12.001

M3 - Article

C2 - 24533316

VL - 2

SP - 54

EP - 61

JO - International Journal For Parasitology: Parasites And Wildlife

JF - International Journal For Parasitology: Parasites And Wildlife

SN - 2213-2244

IS - 1

ER -