Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australian vineyard soils

Gyongyver Korosi, Bree Wilson, Kevin S Powell, Gavin Ash, Annette Reineke, Sandra Savocchia

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Abstract

Entomopathogenic Ascomycetes: Hypocreales fungi occur worldwide in the soil; however, the abundance and distribution of these fungi in a vineyard environment is unknown. A survey of Australian vineyards was carried out in order to isolate and identify entomopathogenic fungi. A total of 240 soil samples were taken from eight vineyards in two states (New South Wales and Victoria). Insect baiting (using Tenebrio molitor) and soil dilution methods were used to isolate Beauveria spp. and Metarhizium spp. from all soil samples. Of the 240 soil samples, 60% contained either Beauveria spp. (26%) or Metarhizium spp. (33%). Species of Beauveria and Metarhizium were identified by sequencing the B locus nuclear intergenic region (Bloc) and elongation factor-1 alpha (EFT1) regions, respectively. Three Beauveria species (B. bassiana, B. australis and B. pseudobassiana) and six Metarhizium species (M. guizhouense, M. robertsii, M. brunneum, M. flavoviride var. pemphigi, M. pingshaense and M. majus) were
identified. A new sister clade made up of six isolates was identified within B. australis. Two potentially new phylogenetic species (six isolates each) were found within the B. bassiana clade. This study revealed a diverse
community of entomopathogenic fungi in sampled Australian vineyard soils.
Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalJournal of Invertebrate Pathology
Volume164
Publication statusPublished - 2019

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Beauveria
Metarhizium
vineyard soils
entomopathogenic fungi
vineyard
vineyards
fungus
soil sampling
Metarhizium brunneum
Victoria (Australia)
soil
Metarhizium flavoviride
Hypocreales
Tenebrio molitor
fungi
baiting
Beauveria bassiana
intergenic DNA
Ascomycota
New South Wales

Cite this

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title = "Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australian vineyard soils",
abstract = "Entomopathogenic Ascomycetes: Hypocreales fungi occur worldwide in the soil; however, the abundance and distribution of these fungi in a vineyard environment is unknown. A survey of Australian vineyards was carried out in order to isolate and identify entomopathogenic fungi. A total of 240 soil samples were taken from eight vineyards in two states (New South Wales and Victoria). Insect baiting (using Tenebrio molitor) and soil dilution methods were used to isolate Beauveria spp. and Metarhizium spp. from all soil samples. Of the 240 soil samples, 60{\%} contained either Beauveria spp. (26{\%}) or Metarhizium spp. (33{\%}). Species of Beauveria and Metarhizium were identified by sequencing the B locus nuclear intergenic region (Bloc) and elongation factor-1 alpha (EFT1) regions, respectively. Three Beauveria species (B. bassiana, B. australis and B. pseudobassiana) and six Metarhizium species (M. guizhouense, M. robertsii, M. brunneum, M. flavoviride var. pemphigi, M. pingshaense and M. majus) wereidentified. A new sister clade made up of six isolates was identified within B. australis. Two potentially new phylogenetic species (six isolates each) were found within the B. bassiana clade. This study revealed a diversecommunity of entomopathogenic fungi in sampled Australian vineyard soils.",
keywords = "Ascomycetes: Hypocreales, Soil survey, vineyard, molecular taxonomy, EFT1",
author = "Gyongyver Korosi and Bree Wilson and Powell, {Kevin S} and Gavin Ash and Annette Reineke and Sandra Savocchia",
year = "2019",
language = "English",
volume = "164",
pages = "69--77",
journal = "Journal of Invertebrate Pathology",
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Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australian vineyard soils. / Korosi, Gyongyver; Wilson, Bree; Powell, Kevin S; Ash, Gavin; Reineke, Annette; Savocchia, Sandra.

In: Journal of Invertebrate Pathology, Vol. 164, 2019, p. 69-77.

Research output: Contribution to journalArticle

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T1 - Occurrence and diversity of entomopathogenic fungi (Beauveria spp. and Metarhizium spp.) in Australian vineyard soils

AU - Korosi, Gyongyver

AU - Wilson, Bree

AU - Powell, Kevin S

AU - Ash, Gavin

AU - Reineke, Annette

AU - Savocchia, Sandra

PY - 2019

Y1 - 2019

N2 - Entomopathogenic Ascomycetes: Hypocreales fungi occur worldwide in the soil; however, the abundance and distribution of these fungi in a vineyard environment is unknown. A survey of Australian vineyards was carried out in order to isolate and identify entomopathogenic fungi. A total of 240 soil samples were taken from eight vineyards in two states (New South Wales and Victoria). Insect baiting (using Tenebrio molitor) and soil dilution methods were used to isolate Beauveria spp. and Metarhizium spp. from all soil samples. Of the 240 soil samples, 60% contained either Beauveria spp. (26%) or Metarhizium spp. (33%). Species of Beauveria and Metarhizium were identified by sequencing the B locus nuclear intergenic region (Bloc) and elongation factor-1 alpha (EFT1) regions, respectively. Three Beauveria species (B. bassiana, B. australis and B. pseudobassiana) and six Metarhizium species (M. guizhouense, M. robertsii, M. brunneum, M. flavoviride var. pemphigi, M. pingshaense and M. majus) wereidentified. A new sister clade made up of six isolates was identified within B. australis. Two potentially new phylogenetic species (six isolates each) were found within the B. bassiana clade. This study revealed a diversecommunity of entomopathogenic fungi in sampled Australian vineyard soils.

AB - Entomopathogenic Ascomycetes: Hypocreales fungi occur worldwide in the soil; however, the abundance and distribution of these fungi in a vineyard environment is unknown. A survey of Australian vineyards was carried out in order to isolate and identify entomopathogenic fungi. A total of 240 soil samples were taken from eight vineyards in two states (New South Wales and Victoria). Insect baiting (using Tenebrio molitor) and soil dilution methods were used to isolate Beauveria spp. and Metarhizium spp. from all soil samples. Of the 240 soil samples, 60% contained either Beauveria spp. (26%) or Metarhizium spp. (33%). Species of Beauveria and Metarhizium were identified by sequencing the B locus nuclear intergenic region (Bloc) and elongation factor-1 alpha (EFT1) regions, respectively. Three Beauveria species (B. bassiana, B. australis and B. pseudobassiana) and six Metarhizium species (M. guizhouense, M. robertsii, M. brunneum, M. flavoviride var. pemphigi, M. pingshaense and M. majus) wereidentified. A new sister clade made up of six isolates was identified within B. australis. Two potentially new phylogenetic species (six isolates each) were found within the B. bassiana clade. This study revealed a diversecommunity of entomopathogenic fungi in sampled Australian vineyard soils.

KW - Ascomycetes: Hypocreales

KW - Soil survey

KW - vineyard

KW - molecular taxonomy

KW - EFT1

UR - https://www.sciencedirect.com/science/article/pii/S0022201118301563

M3 - Article

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JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

SN - 0022-2011

ER -