Optimisation of conditions for the preparation of B-carotene nanoemulsion using response surface methodology

Yuan Yuan, Yanxiang Gao, Like Mao, Jian Zhao

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Response surface methodology (RSM) was used to optimise the conditions for preparing ß-carotene nanoemulsions. The effects of ß-carotene (0.2'1.8%, w/w) and emulsifier (6.9'13.1%, w/w) concentrations, the homogenization pressure (79.1'140.9 MPa) and temperature (34.5'65.5 °C) on the particle size and stability of the nanoemulsions were studied. Results showed that the experimental data could be adequately fitted into a second-order polynomial model with multiple regression coefficients (R2) of 0.921 and 0.981 for the particle size and stability, respectively. Homogenization pressure and ß-carotene concentration and the quadrics of ß-carotene concentration (P<0.05) had a significant effect on the particle size of the nanoemulsions. Homogenization temperature and pressure, ß-carotene concentration, the quadrics of emulsifier concentration and the interactions between ß-carotene and emulsifier concentrations and between homogenization temperature and emulsifier concentration (P<0.05) had a significant effect on the stability of the emulsions. The optimum conditions for preparing ß-carotene nanoemulsions were predicted to be: homogenization pressure, 129 MPa; homogenization temperature, 47 °C; ß-carotene concentration, 0.82%; emulsifier concentration, 8.2%.
Original languageEnglish
Pages (from-to)1300-1306
Number of pages7
JournalFood Chemistry
Volume107
Issue number3
DOIs
Publication statusPublished - 2008

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