Electrostatic interaction between beta-lactoglobulin (β-Lg) and water-soluble fraction of Farsi gum (WSFPG) was studied under the influence of pH, β-Lg:WSFPG mixing ratio (MR) and total concentration (TC) through performing various experiments such as turbidity, particle size and zeta potential measurements. Changes in the relative viscosity during in-situ acidification were studied at various ionic strengths. Lower amounts of absorbance and sedimentation were observed by decreasing the MR. An increase in the TC increased the absorbance of complexed samples. The smallest particle sizes, obtained at a same MR (1:3), were 108 and 188 nm at TCs of 0.1 and 0.3 wt%, respectively. The decreased relative viscosity upon acidification was a proof for the wrapping phenomenon in the biopolymer mixture. A further decrease in the relative viscosity was observed at higher ionic strength. The results of this study may facilitate the rational design of functional nanoparticles in associative mixtures of β-Lg and WSFPG.
|Number of pages||7|
|Journal||International Journal of Biological Macromolecules|
|Early online date||May 2017|
|Publication status||Published - 01 Sep 2017|