This study examined osmotic adjustment (OA) expression and solutes accumulated in leaf segments of wheatcultivar Hartog (with high OA capacity) exposed to polyethylene glycol (PEG). Osmotic adjustment (OA) is one ofthe traits that contribute to plant tolerance of water deficits. The objectives were to evaluate OA expression asresponse to increasing PEG concentration and treatment periods, and to examine the concentrations of inorganicand organic solutes in the tissues, as related to OA development at different duration of PEG treatments. Theexperiment consisted of 4 PEG treatments (0, -0.5, -1.0 and -1.5 MPa), 5 sampling times (0, 12, 24, 48 and 72 h)and 3 replicates. PEG treatments were imposed as -0.5 MPa steps, every 12 h. The leaf segments were sampled forleaf water content, osmotic potential, OA, inorganic solutes (K+, Na+ and Cl-), and organic solutes (glycinebetaine,proline and total soluble sugars). OA was expressed in leaf segments exposed to water deficit under PEG -0.5 MPa,but not at -1.0 MPa or higher. The differences of measurements in PEG -0.5 MPa compared to PEG -1.0 and -1.5MPa, were clearly observed at 48 h. Amongst the solutes, Na+ and proline contributed to leaf OA.
|Number of pages||6|
|Journal||Jurnal Matematika and Sains (Journal of Mathematics and Science)|
|Publication status||Published - Jan 2011|