TY - JOUR
T1 - Overexpression of tumor suppressor gene ZNF750 inhibitsoral squamous cell carcinoma metastasis
AU - Yang, Hongli
AU - Pan, Li
AU - Xu, Cong
AU - Zhang, Yingxin
AU - Li, Keyi
AU - Chen, Shuangfeng
AU - Zhang, Bin
AU - Liu, Zhijun
AU - Wang, Le Xin
AU - Chen, Haiying
N1 - Includes bibliographical references.
PY - 2017/11
Y1 - 2017/11
N2 - Zinc-finger protein 750 (ZNF750) encodes a putative C2H2 zinc finger protein and is typically mutated or deleted in squamous cell carcinoma. The role of ZNF750 in oral squamous cell carcinoma (OSCC) remains unknown. The aim of the present study was to investigate the effects of ZNF750 overexpression in CAL-27 cells. Cell viability, and the expression of genes associated with proliferation, differentiation and the epithelial-mesenchymal transition were investigated in CAL-27 cells following ZNF750 overexpression, using Cell Counting kit-8, reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. In addition, scratch wound, invasion and migration assays were performed. Cell viability, matrix metalloproteinase 28 expression, cyclin B1 expression and mesenchymal marker neural cadherin expression were decreased following ZNF750 overexpression compared with the control groups. ZNF750 overexpression induced the differentiation-associated genes late cornified envelope 3A and small proline-rich protein 1A and upregulated the expression of late epidermal differentiation factor Kruppel-like factor 4. Overexpression of ZNF750 in CAL-27 cells resulted in inhibition of cell invasion and migration. Taken together, these data suggest that ZNF750 may inhibit the metastasis of OSCC.
AB - Zinc-finger protein 750 (ZNF750) encodes a putative C2H2 zinc finger protein and is typically mutated or deleted in squamous cell carcinoma. The role of ZNF750 in oral squamous cell carcinoma (OSCC) remains unknown. The aim of the present study was to investigate the effects of ZNF750 overexpression in CAL-27 cells. Cell viability, and the expression of genes associated with proliferation, differentiation and the epithelial-mesenchymal transition were investigated in CAL-27 cells following ZNF750 overexpression, using Cell Counting kit-8, reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. In addition, scratch wound, invasion and migration assays were performed. Cell viability, matrix metalloproteinase 28 expression, cyclin B1 expression and mesenchymal marker neural cadherin expression were decreased following ZNF750 overexpression compared with the control groups. ZNF750 overexpression induced the differentiation-associated genes late cornified envelope 3A and small proline-rich protein 1A and upregulated the expression of late epidermal differentiation factor Kruppel-like factor 4. Overexpression of ZNF750 in CAL-27 cells resulted in inhibition of cell invasion and migration. Taken together, these data suggest that ZNF750 may inhibit the metastasis of OSCC.
KW - Cell differentiation
KW - Kruppel-like factor 4
KW - Oral squamous cell carcinoma
KW - Proliferation
KW - Zinc-finger protein 750
UR - http://www.scopus.com/inward/record.url?scp=85030168825&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85030168825&partnerID=8YFLogxK
U2 - 10.3892/ol.2017.6908
DO - 10.3892/ol.2017.6908
M3 - Article
C2 - 29113187
AN - SCOPUS:85030168825
SN - 1792-1074
VL - 14
SP - 5591
EP - 5596
JO - Oncology Letters
JF - Oncology Letters
IS - 5
ER -
