TY - JOUR
T1 - Pakistan’ın Subtropikal Bölgelerinde Sığır Hypodermozisinin (Hypoderma lineatum) Tespit Edilmesinde İndirek ELİSA Yönteminin Kullanılması
AU - Ahmed, Haroon
AU - Panadero-Fontán, Rosario
AU - López Sández, Ceferino
AU - Khan, Mobushir Riaz
AU - Asif, Saira
AU - Mustaf, Irfan
AU - Ghani, Abdul
AU - Ahmad, Iftikhar
AU - Naqvi, Syed Muhammad Saqlan
AU - Qayyum, Mazhar
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/11
Y1 - 2013/11
N2 - The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L1) of the warble fy were collected from the surrounding abattoirs to obtain the antigen (HyC) used for development of indirect ELISA. The seroprevalence was 17.4% (174/1000). The highest seroprevalence was recorded in the Rawalpindi district (28.81%), followed by Attock (21.51%), Jhelum (10%) and Chakwal (6.66%). In present study the sensitivity and specificity was 97.28% and 96.44%, respectively. The epidemiological factors showed that the village having hilly locations, local breed, young animals, water bodies, extensive grazing, primary exposed and non medicated were more seropositive as compare to others (P<0.05). In conclusion, indirect ELISA developed during this study is very useful tool for early detection of bovine hypodermosis in cattle grazing in subtropical region of Pakistan.
AB - The aim of this study was to develop an indirect ELISA for the detection of Hypoderma lineatum antibodies and to determine the influence of seroepidemiological factors on the seroprevalence of bovine hypodermosis in cattle of subtropical region of Pakistan. For this purpose a total of 1000 blood samples were taken from twenty eight villages of Rawalpindi, Attock, Chakwal and Jhelum districts. First instar larvae (L1) of the warble fy were collected from the surrounding abattoirs to obtain the antigen (HyC) used for development of indirect ELISA. The seroprevalence was 17.4% (174/1000). The highest seroprevalence was recorded in the Rawalpindi district (28.81%), followed by Attock (21.51%), Jhelum (10%) and Chakwal (6.66%). In present study the sensitivity and specificity was 97.28% and 96.44%, respectively. The epidemiological factors showed that the village having hilly locations, local breed, young animals, water bodies, extensive grazing, primary exposed and non medicated were more seropositive as compare to others (P<0.05). In conclusion, indirect ELISA developed during this study is very useful tool for early detection of bovine hypodermosis in cattle grazing in subtropical region of Pakistan.
KW - Cattle
KW - Hypoderma lineatum
KW - Hypodermosis
KW - Indirect ELISA
KW - Pakistan
KW - Seroprevalence
KW - Subtropical region
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UR - http://vetdergikafkas.org/static.php?id=7
U2 - 10.9775/kvfd.2013.9348
DO - 10.9775/kvfd.2013.9348
M3 - Article
AN - SCOPUS:84884631857
SN - 1300-6045
VL - 19
SP - 1017
EP - 1022
JO - Kafkas Universitesi Veteriner Fakultesi Dergisi
JF - Kafkas Universitesi Veteriner Fakultesi Dergisi
IS - 6
ER -