Pathogenicity, diversity, biology and sources of resistance to Pseudomonas fuscovaginae in rice

The use of a pin-prick method is the most appropriate means to identify sources of resistance, while the spray inoculation method was more suitable for mass screening. The seed-soaking method showed potential in early disease resistance detection. All rice growth stages were susceptible to the pathogen and 107 cfu mL-1 inoculum concentration was optimal for the production of disease symptoms. For the pin-prick method, a single assessment at 14 days post-inoculation could be used to classify genotypes. An index of reduction in seedling height is proposed for use to classify genotypesâÂ' reaction to the disease. The resistant varieties identified here could be used in disease resistance breeding programs. There could be different mechanisms of resistance to P. fuscovaginae in rice based on growth stage. This information will help guide cultivar improvement efforts.