Peptides derived from lupin proteins confer potent protection against oxidative stress

Xiaojuan Guo, Wenting Shang, Padraig Strappe, Zhongkai Zhou, Chris Blanchard

Research output: Contribution to journalArticle

Abstract

Lupin seeds are rich in proteins, which are utilized in the food industry. There is an increased interest in lupin research due to its association with health-related benefits, such as reduction of hypertension and hyperglycemia. However, studies on the peptides derived from lupin proteins are rare. RESULTS: Lupin protein hydrolysates (LPHs) were prepared by proteolysis using alcalase, trypsin and pepsin, respectively. All the hydrolysates demonstrated higher antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities compared to lupin proteins. The hydrolysates were fractionated into three fractions based on molecular weight (MW), and the peptides with MW < 3 kDa (LPH3) had the highest antioxidant and ACE inhibitory activities compared to other fractions. Cell model study revealed that LPH3 fraction had the highest protection against the generation of reactive oxygen species in HepG2 cells, which was associated with increased activities of superoxide dismutase and glutathione peroxidase through upregulation of SOD1, GPX1, GCLM, SLC7A11 and SRXN1 expression. CONCLUSIONS: The analysis of amino acid composition indicated that the peptides were characterized with high content of hydrophobic amino acids, which may be responsible for the greatest antioxidant activity. This study highlights the promising potential of lupin peptides as a functional ingredient in healthy foods.
Original languageEnglish
Pages (from-to)5225-5234
Number of pages10
JournalJournal of the Science of Food and Agriculture
Volume98
Issue number14
Early online date10 Apr 2018
DOIs
Publication statusPublished - 01 Nov 2018

Fingerprint

Lupinus
Oxidative Stress
oxidative stress
peptides
Peptides
Antioxidants
Peptidyl-Dipeptidase A
Proteins
proteins
Molecular Weight
peptidyl-dipeptidase A
Subtilisins
Protein Hydrolysates
hydrolysates
Amino Acids
Pepsin A
Food Industry
Hep G2 Cells
Insurance Benefits
Glutathione Peroxidase

Cite this

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abstract = "Lupin seeds are rich in proteins, which are utilized in the food industry. There is an increased interest in lupin research due to its association with health-related benefits, such as reduction of hypertension and hyperglycemia. However, studies on the peptides derived from lupin proteins are rare. RESULTS: Lupin protein hydrolysates (LPHs) were prepared by proteolysis using alcalase, trypsin and pepsin, respectively. All the hydrolysates demonstrated higher antioxidant and angiotensin I-converting enzyme (ACE) inhibitory activities compared to lupin proteins. The hydrolysates were fractionated into three fractions based on molecular weight (MW), and the peptides with MW < 3 kDa (LPH3) had the highest antioxidant and ACE inhibitory activities compared to other fractions. Cell model study revealed that LPH3 fraction had the highest protection against the generation of reactive oxygen species in HepG2 cells, which was associated with increased activities of superoxide dismutase and glutathione peroxidase through upregulation of SOD1, GPX1, GCLM, SLC7A11 and SRXN1 expression. CONCLUSIONS: The analysis of amino acid composition indicated that the peptides were characterized with high content of hydrophobic amino acids, which may be responsible for the greatest antioxidant activity. This study highlights the promising potential of lupin peptides as a functional ingredient in healthy foods.",
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Peptides derived from lupin proteins confer potent protection against oxidative stress. / Guo, Xiaojuan; Shang, Wenting; Strappe, Padraig; Zhou, Zhongkai; Blanchard, Chris.

In: Journal of the Science of Food and Agriculture, Vol. 98, No. 14, 01.11.2018, p. 5225-5234.

Research output: Contribution to journalArticle

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